SOX17 was detected in immersion fixed endoderm-differentiated BG01V human embryonic stem cells using Mouse Anti-Human SOX17 Monoclonal Antibody (Catalog # MAB19241) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (yellow, upper panel; NL007) and counterstained with DAPI (blue, lower panel). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Detection of SOX17 in Endoderm Differentiated BG01V hESCs by Flow Cytometry

Endoderm differentiated BG01V hESCs were stained with Mouse Anti-Human SOX17 Monoclonal Antibody (Catalog # MAB19241, filled histogram) or isotype control antibody (Catalog # MAB0041, open histogram) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules. Endoderm differentiation was performed using SC019B following protocol on the datasheet.

Detection of SOX17 in iPSCs by Flow Cytometry

iPSCs were stained with Mouse Anti-Human SOX17 Monoclonal Antibody (Catalog # MAB19241, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.

Applications for Human SOX17 Antibody

Application

Recommended Usage

Immunocytochemistry

8-25 µg/mL
Sample: Immersion-fixed endoderm-differentiated BG01V human embryonic stem cells

Intracellular Staining by Flow Cytometry

0.25 µg/10⁶ cells
Sample: Endoderm-differentiated BG01V human embryonic stem cells; iPSCs

Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: SOX17

SOX17 is a member of the SOX family of transcription factors that bind DNA via a high mobility group (HMG) domain. SOX17 is suggested to play an important role in endoderm development (1, 2).

References

Kanai-Azuma, M. et al. (2002) Development 129:2367.
Katoh, M. et al. (2002) Int. J. Mol. Med. 9:153.

Long Name

Transcription Factor SOX17

Alternate Names

FLJ22252, SRY (sex determining region Y)-box 17, SRY-related HMG-box transcription factor SOX17, transcription factor SOX-17, VUR3

Entrez Gene IDs

64321 (Human)

Gene Symbol

SOX17

UniProt

Q9H6I2

Additional SOX17 Products

Product Documents for Human SOX17 Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Human SOX17 Antibody

For research use only

Citations
Reviews

Calculators
Flow Cytometry

Human SOX17 Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB19241

Key Product Details

Species Reactivity

Validated:

Cited:

Applications

Validated:

Cited:

Label

Antibody Source

View all SOX17 Antibodies »

Product Specifications

Immunogen

Specificity

Clonality

Host

Isotype

Scientific Data Images for Human SOX17 Antibody

SOX17 in BG01V Human Stem Cells.