Human SOX18 Antibody
R&D Systems, part of Bio-Techne | Catalog # AF5077
Key Product Details
Species Reactivity
Human
Applications
Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Sheep IgG
Product Specifications
Immunogen
E. coli-derived recombinant human SOX18
Ala276-Gly384
Accession # P35713
Ala276-Gly384
Accession # P35713
Specificity
Detects endogenous human SOX18 in Western blots.
Clonality
Polyclonal
Host
Sheep
Isotype
IgG
Scientific Data Images for Human SOX18 Antibody
Detection of Human SOX18 by Western Blot.
Western blot shows lysates of Jurkat human acute T cell leukemia cell line, HUVEC human umbilical vein endothelial cells, and K562 human chronic myelogenous leukemia cell line. PVDF membrane was probed with 1 µg/mL of Human SOX18 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5077) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for SOX18 at approximately 41 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.Applications for Human SOX18 Antibody
Application
Recommended Usage
Western Blot
1 µg/mL
Sample: Jurkat human acute T cell leukemia cell line, HUVEC human umbilical vein endothelial cells, and K562 human chronic myelogenous leukemia cell line
Sample: Jurkat human acute T cell leukemia cell line, HUVEC human umbilical vein endothelial cells, and K562 human chronic myelogenous leukemia cell line
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: SOX18
SOX18 (SRY-related HMG box) is a 41 kDa (predicted) Group F member of the SOX family of DNA-binding proteins. Human SOX18 is 384 amino acids (aa) in length and contains an N-terminal DNA-binding HMG box (aa 85‑153), a central TAD region (aa 168‑260) and a highly charged sequence that contains a nine aa transactivation motif (aa 323‑331). SOX18 is expressed by endothelium and vascular smooth muscle, and apparently promotes the formation and organization of new capillaries. Over aa 276‑384, human SOX18 shares 90% and 89% aa identity with mouse and porcine SOX18, respectively.
Long Name
SRY-related HMG-box 18
Alternate Names
HLTS
Gene Symbol
SOX18
UniProt
Additional SOX18 Products
Product Documents for Human SOX18 Antibody
Product Specific Notices for Human SOX18 Antibody
For research use only
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