Human SOX3 Antibody
R&D Systems, part of Bio-Techne | Catalog # AF2569
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Mouse
Applications
Validated:
Immunocytochemistry, Western Blot
Cited:
Flow Cytometry, Immunocytochemistry, Immunohistochemistry, Immunohistochemistry-Frozen, Immunoprecipitation
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Product Specifications
Immunogen
E. coli-derived recombinant human SOX3
Met1-Leu230
Accession # P41225
Met1-Leu230
Accession # P41225
Specificity
Detects human SOX3 in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than 1% cross-reactivity with recombinant human (rh) SOX2, rhSOX7, rhSOX10, and rhSOX17 is observed.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for Human SOX3 Antibody
SOX3 in NTera‑2 Human Cell Line.
SOX3 was detected in immersion fixed NTera-2 human testicular embryonic carcinoma cell line differentiation with retinoic acid using Goat Anti-Human SOX3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2569) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 493-conjugated Anti-Goat IgG Secondary Antibody (green, upper panel; NL003) and counterstained with DAPI (blue, lower panel). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Detection of SOX3 in U-251 MG cells (positive) and HDLM-2 cells (negative).
SOX3 was detected in immersion fixed U-251 MG human glioblastoma cells (positive) and absent in HDLM-2 human Hodgkin’s lymphoma cells (negative) using Goat Anti-Human SOX3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2569) at 15 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to cell nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.Applications for Human SOX3 Antibody
Application
Recommended Usage
Immunocytochemistry
5-15 µg/mL
Sample: Immersion fixed NTera-2 human testicular embryonic carcinoma cell line, U-251 MG human glioblastoma cells (positive) and HDLM-2 human Hodgkin's lymphoma cells (negative)
Sample: Immersion fixed NTera-2 human testicular embryonic carcinoma cell line, U-251 MG human glioblastoma cells (positive) and HDLM-2 human Hodgkin's lymphoma cells (negative)
Western Blot
0.1 µg/mL
Sample: Recombinant Human SOX3
Sample: Recombinant Human SOX3
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: SOX3
Long Name
Transcription Factor SOX3
Alternate Names
SOXB
Entrez Gene IDs
6658 (Human)
Gene Symbol
SOX3
UniProt
Additional SOX3 Products
Product Documents for Human SOX3 Antibody
Product Specific Notices for Human SOX3 Antibody
For research use only
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