In Western blots, approximately 5% cross-reactivity with recombinant human (rh) SOX10 is observed and less than 2% cross-reactivity with rhSOX2, rhSOX3, rhSOX7, rhSOX14, rhSOX17, and rhSOX21 is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Human SOX9 Biotinylated Antibody

SOX9 in BGO1V Human Embryonic Stem Cells.

SOX9 was detected in immersion fixed BGO1V human embryonic stem cells differentiated to early proximal lung progenitors using Goat Anti-Human SOX9 Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF3075) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Streptavidin (red; Catalog # NL999) and counterstained with DAPI (blue). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Stem Cells on Coverslips.

Applications for Human SOX9 Biotinylated Antibody

Application

Recommended Usage

Immunocytochemistry

5-15 µg/mL
Sample: Immersion fixed human mesenchymal stem cells differentiated to osteocytes, adipocytes, and chondrocytes using Human Mesenchymal Stem Cell Functional Identification Kit (Catalog # SC006) and immersion fixed BG01V human embryonic stem cells differentiated to early proximal lung progenitor

Western Blot

0.1 µg/mL
Sample: Recombinant Human SOX9

Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: SOX9

SOX9 belongs to the SOX (SRY-like HMG box) family of transcription factors with diverse roles in development. SOX9 is expressed in mesenchymal progenitors that give rise to chondrocytes and osteoblasts. It is also expressed in the central nervous system, neural crest, intestine, pancreas, and testis. Mutations in SOX9 are associated with defects in sex determination, cartilage and bone development, as well as abnormalities of the heart, kidneys, brain, gut, and pancreas.

Long Name

Transcription Factor SOX9

Alternate Names

campomelic dysplasia, autosomal sex-reversal, CMD 1, CMD1, CMPD1, SRA1SRY (sex-determining region Y)-box 9 protein, SRY (sex determining region Y)-box 9, SRY-related HMG-box, gene 9, transcription factor SOX-9

Entrez Gene IDs

6662 (Human)

Gene Symbol

SOX9

UniProt

P48436

Additional SOX9 Products

Product Documents for Human SOX9 Biotinylated Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Human SOX9 Biotinylated Antibody

For research use only

Citations
Reviews

Human SOX9 Biotinylated Antibody

R&D Systems, part of Bio-Techne | Catalog # BAF3075

Key Product Details

Species Reactivity

Applications

Label

Antibody Source

View all SOX9 Antibodies »

Product Specifications

Immunogen

Specificity