Skip to main content

Human Spinesin Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB2495

R&D Systems, part of Bio-Techne
Catalog #
Availability
Size / Price
Qty
Loading...
MAB2495
MAB2495-SP

Key Product Details

Species Reactivity

Human

Applications

Immunoprecipitation, Neutralization

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # 376608

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant human Spinesin
Tyr27-Leu413
Accession # Q0P514

Specificity

Detects human Spinesin in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant mouse Spinesin is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Applications for Human Spinesin Antibody

Application
Recommended Usage

Immunoprecipitation

25 µg/mL
Sample: Conditioned cell culture medium spiked with Recombinant Human Spinesin (Catalog # 2495‑SE), see our available Western blot detection antibodies

Neutralization

Measured by its ability to neutralize Recombinant Human Spinesin (1 µg/mL, Catalog # 2495-SE) cleavage of the fluorogenic peptide substrate Boc-QAR-Amc (0.1 mM, Catalog # ES014). The Neutralization Dose (ND50) is typically 7 µg/mL.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Reconstitution Buffer Available:
Size / Price
Qty
Loading...

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Spinesin

Spinesin, encoded by the TMPRSS5 gene, is a member of type II transmembrane serine proteases (TTSPs) (1). Human Spinesin contains the following structural domains: a short N‑terminal cytoplasmic tail (amino acid residues 1‑49), a transmembrane domain (residues 50‑70), a stem region and a serine protease domain (residues 71‑457) (2). The domain structure of Spinesin is common to other TTSPs, many of which have additional domains. The stem region of Spinesin contains a scavenger receptor-like domain. The ectodomain of human Spinesin (residues 71‑457) was expressed and purified as a single chain pro-enzyme. The deduced amino acid sequence contains a Leu instead of a Phe residue at position 369; the former is identical to the mouse protein (3, 4). The pro-enzyme can be activated and the resulting enzyme activity can be measured as described in the Activity Assay Protocol.

References

  1. Hooper, J.D. et al. (2001) J. Biol. Chem. 276:857.
  2. Yamaguchi, Y. et al. (2002) J. Biol. Chem. 277:6806.
  3. Carninci, P. et al. (2000) Genome Res. 10:1617.
  4. Shibata, K. et al. (2000) Genome Res. 10:1757.

Alternate Names

TMPRSS5

Entrez Gene IDs

80975 (Human); 80893 (Mouse)

Gene Symbol

TMPRSS5

UniProt

Additional Spinesin Products

Product Documents for Human Spinesin Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Human Spinesin Antibody

For research use only

Loading...
Loading...
Loading...
Loading...