Human Spinesin Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB2495
Key Product Details
Species Reactivity
Applications
Label
Antibody Source
Product Specifications
Immunogen
Tyr27-Leu413
Accession # Q0P514
Specificity
Clonality
Host
Isotype
Applications for Human Spinesin Antibody
Immunoprecipitation
Sample: Conditioned cell culture medium spiked with Recombinant Human Spinesin (Catalog # 2495‑SE), see our available Western blot detection antibodies
Formulation, Preparation, and Storage
Purification
Reconstitution
Formulation
Shipping
Stability & Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Spinesin
Spinesin, encoded by the TMPRSS5 gene, is a member of type II transmembrane serine proteases (TTSPs) (1). Human Spinesin contains the following structural domains: a short N‑terminal cytoplasmic tail (amino acid residues 1‑49), a transmembrane domain (residues 50‑70), a stem region and a serine protease domain (residues 71‑457) (2). The domain structure of Spinesin is common to other TTSPs, many of which have additional domains. The stem region of Spinesin contains a scavenger receptor-like domain. The ectodomain of human Spinesin (residues 71‑457) was expressed and purified as a single chain pro-enzyme. The deduced amino acid sequence contains a Leu instead of a Phe residue at position 369; the former is identical to the mouse protein (3, 4). The pro-enzyme can be activated and the resulting enzyme activity can be measured as described in the Activity Assay Protocol.
References
- Hooper, J.D. et al. (2001) J. Biol. Chem. 276:857.
- Yamaguchi, Y. et al. (2002) J. Biol. Chem. 277:6806.
- Carninci, P. et al. (2000) Genome Res. 10:1617.
- Shibata, K. et al. (2000) Genome Res. 10:1757.
Alternate Names
Gene Symbol
UniProt
Additional Spinesin Products
Product Documents for Human Spinesin Antibody
Product Specific Notices for Human Spinesin Antibody
For research use only