Human SPRED1 Antibody
R&D Systems, part of Bio-Techne | Catalog # AF5067
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human
Applications
Validated:
Western Blot
Cited:
Immunocytochemistry, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Sheep IgG
Product Specifications
Immunogen
E. coli-derived recombinant human SPRED1
Arg118-Lys324
Accession # Q7Z699
Arg118-Lys324
Accession # Q7Z699
Specificity
Detects endogenous human SPRED1 in Western blots.
Clonality
Polyclonal
Host
Sheep
Isotype
IgG
Scientific Data Images for Human SPRED1 Antibody
Detection of Human SPRED1 by Western Blot.
Western blot shows lysates of K562 human chronic myelogenous leukemia cell line and human brain tissue. PVDF membrane was probed with 1 µg/mL of Human SPRED1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5067) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for SPRED1 at approximately 55 kDa (as indicated). This experiment was conducted using Immunoblot Buffer Group 1.Applications for Human SPRED1 Antibody
Application
Recommended Usage
Western Blot
1 µg/mL
Sample: K562 human chronic myelogenous leukemia cell line and human brain tissue
Sample: K562 human chronic myelogenous leukemia cell line and human brain tissue
Reviewed Applications
Read 2 reviews rated 3.5 using AF5067 in the following applications:
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: SPRED1
SPRED1 (Sprouty-related protein with an EVH1 domain-1) is a 55 kDa member of the SPRED family of regulatory molecules. It inhibits mitogenic signaling by blocking Ras activation of Raf, and disrupts actin stress fiber formation by binding TESK1. Human SPRED1 is 444 amino acids (aa) in length. It contains an N‑terminal WH1/EVH1 domain that is involved in ERK inhibition (aa 6‑123), a central KBD domain that binds to the SCFR (aa 233‑285), and a C-terminal Cys‑rich/Sprouty-related domain that mediates homo- and heterodimerization with SPRED2, binding to TESK1, and undergoes palmitoylation for membrane localization (aa 334‑442). There is one splice variant that shows a nine Lys substitution for aa 269‑444.
Long Name
Sprouty-related with an EVH1 Domain Containing-1
Alternate Names
NFLS
Gene Symbol
SPRED1
UniProt
Additional SPRED1 Products
Product Documents for Human SPRED1 Antibody
Product Specific Notices for Human SPRED1 Antibody
For research use only
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