Human Src Antibody

Detection of Human Src by Western Blot.

Western blot shows lysates of A549 human lung carcinoma cell line, HepG2 human hepatocellular carcinoma cell line, U937 human histiocytic lymphoma cell line (negative control), and HL-60 human acute promyelocytic leukemia cell line (negative control). PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human Src Monoclonal Antibody (Catalog # MAB3807) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (HAF018). A specific band was detected for Src at approximately 60 kDa (as indicated). GAPDH (MAB5718) is shown as a loading control. This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1.

Detection of Human and Mouse Src by Western Blot.

Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line, C2C12 mouse myoblast cell line, and mIMCD-3 mouse epithelial cell line. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human Src Monoclonal Antibody (Catalog # MAB3807) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (HAF018). A specific band was detected for Src at approximately 60 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1.

Src in Human Lung Cancer Tissue.

Src was detected in immersion fixed paraffin-embedded sections of human lung cancer tissue using Mouse Anti-Human Src Monoclonal Antibody (Catalog # MAB3807) at 25 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling if primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.