HT-29 human colon adenocarcinoma cell line was stained with Mouse Anti-Human STRA6 Monoclonal Antibody (Catalog # MAB5904, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG F(ab')₂Secondary Antibody (Catalog # F0101B). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with saponin.

STRA6 in Raji Human Cell Line.

STRA6 was detected in immersion fixed Raji human Burkitt's lymphoma cell line using Mouse Anti-Human STRA6 Monoclonal Antibody (Catalog # MAB5904) at 3 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to plasma membrane and cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Applications for Human STRA6 Antibody

Application

Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Immunocytochemistry

8-25 µg/mL
Sample: Immersion fixed HT-29 human colon adenocarcinoma cell line and Raji human Burkitt's lymphoma cell line

Intracellular Staining by Flow Cytometry

2.5 µg/10⁶ cells
Sample: HT-29 human colon adenocarcinoma cell line fixed with paraformaldehyde and permeabilized with saponin

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: STRA6

Stimulated by retinoic acid gene 6 protein homolog (STRA6) is a 74 kDa broadly expressed transmembrane protein that does not belong to any particular family of proteins. Human STRA6 is 667 amino acids (aa) in length. It contains nine transmembrane regions and one potential N-linked glycosylation site in the first extracellular domain. In addition, there are three splicing variants producing four isoforms. There is no signal sequence. Human STRA6 is 80% identical to bovine STRA6 and 74% identical to mouse and rat STRA6. STRA6 may act as a high-affinity cell-surface receptor for the complex retinol-retinol binding protein (RBP/RBP4). Defects in STRA6 are the cause of Spear syndrome and Matthew-Wood syndrome.

Long Name

Stimulated by Retinoic Acid Gene 6 Protein Homolog

Alternate Names

MCOPS9

Entrez Gene IDs

64220 (Human); 20897 (Mouse)

Gene Symbol

STRA6

UniProt

AAH25256

Additional STRA6 Products

Product Documents for Human STRA6 Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Human STRA6 Antibody

For research use only

Citations
Reviews

Calculators
Flow Cytometry

Human STRA6 Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB5904

Key Product Details

Species Reactivity

Validated:

Cited:

Applications

Validated:

Cited:

Label

Antibody Source

View all STRA6 Antibodies »

Product Specifications

Immunogen

Specificity

Clonality

Host

Isotype

Scientific Data Images for Human STRA6 Antibody

Detection of STRA6 in HT-29 Human Cell Line by Flow Cytometry.