Human Sulfamidase/SGSH Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB8380

R&D Systems, part of Bio-Techne
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MAB8380-100
MAB8380-SP
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Key Product Details

Species Reactivity

Human

Applications

Immunocytochemistry

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # 1018387

View all Sulfamidase/SGSH Antibodies »

Product Specifications

Immunogen

Spodoptera frugiperda, Sf 21 (baculovirus)-derived human Sulfamidase/SGSH protein
Arg23-Leu502
Accession # P51688

Specificity

Detects human Sulfamidase/SGSH in direct ELISAs.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Scientific Data Images for Human Sulfamidase/SGSH Antibody

Sulfamidase/SGSH antibody in HT-29 Human Cell Line by Immunocytochemistry (ICC).

Sulfamidase/SGSH in HT‑29 Human Cell Line.

Sulfamidase/SGSH was detected in immersion fixed HT-29 human colon adenocarcinoma cell line (positive stain) and U937 human histiocytic lymphoma cell line (negative stain) using Mouse Anti-Human Sulfamidase/SGSH Monoclonal Antibody (Catalog # MAB8380) at 8 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Applications for Human Sulfamidase/SGSH Antibody

Application
Recommended Usage

Immunocytochemistry

8-25 µg/mL
Sample: Immersion fixed HT‑29 human colon adenocarcinoma cell line

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Reconstitution Buffer Available:
Size / Price
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Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Sulfamidase/SGSH

Also known as N-sulfoglucosamine sulfohydrolase and heparan N-sulfatase, Sulfamidase/SGSH is an important member of the sulfatase family involved in the degradation of heparan sulfate (HS) (1). Different from the HS specific endosulfatases that remove sulfate from internal GlcNAc residues (2), SGSH removes sulfate group from the non-reducing end glucosamine residues on HS. The SGSH deficiency results in mucopolysaccharidosis type IIIA (MPS IIIA, Sanfilippo A syndrome), an autosomal recessive lysosomal storage disease characterized by neurological dysfunction but relatively mild somatic manifestations (3). Human SGSH shows 88.6% sequence identity with that of mouse sequence.

References

  1. DiezRoux, G. and Ballabio, A. et al. (2005) Annu. Rev. Genomics Hum. Genet. 6:355.
  2. Morimoto-Tomita, M. et al. (2002) J. Biol. Chem. 277:49175.
  3. Blanch, L. et al. (1997) Hum. Mol. Genet. 6:787.

Alternate Names

HSS, MPS3A, SFMD, SGSH

Entrez Gene IDs

6448 (Human); 27029 (Mouse)

Gene Symbol

SGSH

UniProt

P51688

Additional Sulfamidase/SGSH Products

Product Documents for Human Sulfamidase/SGSH Antibody

Product Datasheets

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human Sulfamidase/SGSH Antibody

For research use only

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