Human Synaptophysin Antibody

R&D Systems, part of Bio-Techne | Catalog # AF5555

R&D Systems, part of Bio-Techne
Catalog #
Availability
Size / Price
Qty
AF5555
AF5555-SP
Print Quote
Bulk Order
100% Guarantee

Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human, Mouse, Rat

Applications

Validated:

Immunohistochemistry, Simple Western, Western Blot

Cited:

Immunocytochemistry, Immunohistochemistry, Immunohistochemistry-Frozen, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

View all Synaptophysin Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human Synaptophysin
Glu50-Phe106
Accession # P08247

Specificity

Detects human Synaptophysin in direct ELISAs and Western blots.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Human Synaptophysin Antibody

Synaptophysin antibody in Human Brainstem Tissue by Immunohistochemistry (IHC-P).

Synaptophysin in Human Brainstem Tissue.

Synaptophysin was detected in immersion fixed paraffin-embedded sections of human brainstem tissue using Goat Anti-Human Synaptophysin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5555) at 1.7 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to olivary nucleus. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections. *Not recommended for Immunohistochemistry/Immunocytochemistry on mouse and rat samples.
Detection of Human, Mouse, and Rat Synaptophysin antibody by Western Blot.

Detection of Human, Mouse, and Rat Synaptophysin by Western Blot.

Western blot shows lysates of human brain tissue, mouse brain tissue, and rat brain tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human Synaptophysin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5555) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for Synaptophysin at approximately 38 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Detection of Human Synaptophysin by Simple WesternTM.

Simple Western shows lysates of human cerebellum, loaded at 0.5 mg/ml. A specific band was detected for Synaptophysin at approximately 59 kDa (as indicated) using 50 µg/mL of Goat Anti-Human Synaptophysin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5555). This experiment was conducted under reducing conditions and using the 12-230kDa separation system.

Applications for Human Synaptophysin Antibody

Application
Recommended Usage

Immunohistochemistry

5-15 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human brainstem tissue

Simple Western

50 µg/mL
Sample: Human cerebellum

Western Blot

1 µg/mL
Sample: Human brain tissue, mouse brain tissue, and rat brain tissue
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Reviewed Applications

Read 1 review rated 5 using AF5555 in the following applications:

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Reconstitution Buffer Available:
Size / Price
Qty

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details.
*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Synaptophysin

Synaptophysin (also p38 and BM89 in mouse) is a 38‑40 kDa member of the synaptophysin/synaptobrevin family of proteins. It is expressed in neurons, and interacts with VAMP2 at an early stage of vesicle formation, guiding VAMP2 placement into secretory vesicles that are destined for release upon stimulation. Human Synaptophysin is a four transmembrane protein that is 313 amino acids (aa) in length. It contains two luminal domains (aa 50‑106 and 162‑199) and a cytoplasmic C‑terminal targeting region that specifies its localization (aa 214‑313). Synaptophysin forms homodimers and oligomers, and heterodimerizes with VAMP2. There is one isoform variant that shows a six aa substitution for aa 35‑313. Over aa 50‑106, human Synaptophysin shares 89% aa identity with mouse Synaptophysin.

Alternate Names

SYP

Entrez Gene IDs

6855 (Human)

Gene Symbol

SYP

UniProt

P08247

Additional Synaptophysin Products

Product Documents for Human Synaptophysin Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Human Synaptophysin Antibody

For research use only

Privacy and Cookie Policy
Site Map
© Copyright 2024 Bio-Techne. All Rights Reserved.