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Human Syndecan-2/CD362 Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB2965

Clone 305515 was used by HLDA to establish CD designation
R&D Systems, part of Bio-Techne

Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

CyTOF-ready, Flow Cytometry

Cited:

Flow Cytometry

Label

Unconjugated

Antibody Source

Monoclonal Rat IgG2B Clone # 305515

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant human Syndecan-2/CD362
Glu19-Gly144
Accession # AAH49836.1

Specificity

Detects human Syndecan-2/CD362 in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant mouse Syndecan-1, recombinant human (rh) Syndecan-3, or rhSyndecan-4 is observed.

Clonality

Monoclonal

Host

Rat

Isotype

IgG2B

Scientific Data Images for Human Syndecan-2/CD362 Antibody

Detection of Syndecan-2/CD362 antibody in Human PBMCs antibody by Flow Cytometry.

Detection of Syndecan-2/CD362 in Human PBMCs by Flow Cytometry.

Human peripheral blood mononuclear cells (PBMCs) were stained with Mouse Anti-Human CD14 APC-conjugated Monoclonal Antibody (Catalog # FAB3832A) and either (A) Rat Anti-Human Syndecan-2/CD362 Monoclonal Antibody (Catalog # MAB2965) or (B) Rat IgG2BIsotype Control (Catalog # MAB0061) followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B). View our protocol for Staining Membrane-associated Proteins.

Applications for Human Syndecan-2/CD362 Antibody

Application
Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry

0.25 µg/106 cells
Sample: COLO 205 human colorectal adenocarcinoma cell line

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Reconstitution Buffer Available:
Size / Price
Qty
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Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Syndecan-2/CD362

Syndecan-2, previously known as fibroglycan or heparan sulfate proteoglycan, is a member of the syndecan family of Type 1 transmembrane proteins capable of carrying heparan sulfate (HS) and chondroitin sulfate glycosaminoglycans. The four vertebrate syndecans show conserved cytoplasmic domains and divergent extracellular portions (except for GAG attachment sites). Among the Syndecans, Syndecan-2 is most similar to Syndecan-4 (1‑3). Human Syndecan-2 is synthesized as a 201 amino acid (aa) core protein with an 18 aa signal sequence, a 126 aa extracellular domain (ECD), a 25 aa transmembrane region and a 32 aa cytoplasmic tail (4). The human ECD of Syndecan-2 contains three closely-spaced consensus Ser-Gly sequences for the attachment of HS side chains. It shares 76%, 73%, 87%, 78% and 63% aa identity with the ECD of mouse, rat, bovine, canine and chicken Syndecan-2, respectively. The cytoplasmic tail has both serine and tyrosine phosphorylation sites. Addition of 20‑80 disaccharides per side chain adds considerably to the size of the 22 kDa core protein. Non-covalent homodimerization of Syndecan-2 is dependent on the transmembrane domain (5). Syndecan-2 is expressed in cells of mesenchymal origin, neuronal and epithelial cells, and is the predominant syndecan expressed during embryonic development. Expression is upregulated in several cancer cell lines (6). After induction in macrophages by inflammatory mediators, Syndecan-2 selectively binds FGFbasic, VEGF and EGF (7). Syndecan-2 expressed on human primary osteoblasts binds GM-CSF and may function as a co‑receptor (8). Activated endothelial cell Syndecan-2 specifically binds IL-8 and may participate in promoting neutrophil extravasation by forming a chemotactic IL-8 gradient (9). Typically, cytokine, chemokine and extracellular matrix protein binding occurs through interaction with HS side chains, but the Syndecan-2 extracellular domain can bind TGF-beta directly via protein-protein interaction (10).

References

  1. Tkachenko, E. et al. (2005) Circ. Res. 96:488.
  2. Oh, E.-S, and J. R. Couchman (2004) Mol. Cells 17:181.
  3. Essner, J. J. et al. (2006) Int. J. Biochem. Cell Biol. 38:152.
  4. Marynen, P. et al. (1989) J. Biol. Chem. 264:7017.
  5. Choi, S. et al. (2005) J. Biol. Chem. 280:42573.
  6. Park, H. et al. (2002) J. Biol. Chem. 277:29730.
  7. Clasper, S. et al. (1999) J. Biol. Chem. 274:24113.
  8. Modrowski, D. et al. (2000) J. Biol. Chem. 275:9178.
  9. Halden, Y. et al. (2004) Biochem. J. 377:533.
  10. Chen, L. et al. (2004) J. Biol. Chem. 279:15715.

Alternate Names

CD362, Fibroglycan, HSPG1, SDC2, SYND2, Syndecan2

Entrez Gene IDs

6383 (Human); 15529 (Mouse)

Gene Symbol

SDC2

UniProt

Additional Syndecan-2/CD362 Products

Product Documents for Human Syndecan-2/CD362 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Human Syndecan-2/CD362 Antibody

For research use only

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