Human Syntaxin 6 Biotinylated Antibody

R&D Systems, part of Bio-Techne | Catalog # BAF5664

R&D Systems, part of Bio-Techne
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BAF5664
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AF5664
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Key Product Details

Species Reactivity

Human

Applications

Immunocytochemistry

Label

Biotin

Antibody Source

Polyclonal Sheep IgG

View all Syntaxin 6 Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human Syntaxin 6
Ser69-Asn217
Accession # O43752

Specificity

Syntaxin 6 antibodies are ideal for immunocytochemistry colocalization studies in trans-Golgi and endosomal membranes. The unconjugated antibody detects human Syntaxin 6 in direct ELISAs and Western blots. In direct ELISAs, less than 3% cross‑reactivity with recombinant human (rh) Syntaxin 8, rhSyntaxin 12, and rhSyntaxin 16 is observed.

Clonality

Polyclonal

Host

Sheep

Isotype

IgG

Scientific Data Images for Human Syntaxin 6 Biotinylated Antibody

Syntaxin 6 antibody in HeLa Human Cell Line by Immunocytochemistry (ICC).

Syntaxin 6 in HeLa Human Cell Line.

Syntaxin 6 was detected in formaldehyde fixed HeLa human cervical epithelial carcinoma cell line using Sheep Anti-Human Syntaxin 6 Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF5664) at 15 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Streptavidin (red; Catalog # NL999) and counterstained with DAPI (blue). Specific staining was localized to Golgi bodies. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Applications for Human Syntaxin 6 Biotinylated Antibody

Application
Recommended Usage

Immunocytochemistry

5-15 µg/mL
Sample: Formaldehyde fixed HeLa human cervical epithelial carcinoma cell line

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Reconstitution Buffer Available:
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Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Syntaxin 6

Syntaxin 6 (STX6) is a 30‑35 kDa member of the syntaxin family of proteins. It is widely expressed, embedded in trans‑Golgi and endosomal membranes, and is associated with a variety of SNARE proteins. STX6 is involved in the regulation of caveolae‑dependent endocytosis, and is crucial for membrane remodeling. Human STX6 is a type IV single‑pass transmembrane protein (very long cytoplasmic N‑terminus) that is 255 amino acids (aa) in length. It contains a coiled‑coil region (aa 41‑74), a t‑SNARE domain (aa 163‑225) that is likely involved in protein‑protein interactions, and a C‑terminal transmembrane sequence. There are two splice variants that are also 255 aa in length, but show variation over aa 69‑73. Over aa 69‑217, human STX6 shares 93% aa identity with mouse STX6.

Alternate Names

STX6

Entrez Gene IDs

10228 (Human); 58244 (Mouse); 60562 (Rat)

Gene Symbol

STX6

UniProt

O43752 (Human)

Additional Syntaxin 6 Products

Product Documents for Human Syntaxin 6 Biotinylated Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Human Syntaxin 6 Biotinylated Antibody

For research use only

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