Human Syntaxin 6 Antibody

Name: Human Syntaxin 6 Antibody
Brand: R&D Systems
SKU: AF5664

R&D Systems, part of Bio-Techne | Catalog # AF5664

Submit a review

Product Datasheet / COA / SDS

View Available Conjugates

Conjugate

Catalog #

Biotin

BAF5664

View Product

100% Guarantee

Product Specifications
Scientific Data
Applications
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Species Reactivity

Human

Applications

Immunocytochemistry, Immunohistochemistry, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Sheep IgG

View all Syntaxin 6 Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human Syntaxin 6
Ser69-Asn217
Accession # O43752

Specificity

Detects human Syntaxin 6 in direct ELISAs and Western blots. In direct ELISAs, less than 3% cross‑reactivity with recombinant human (rh) Syntaxin 8, rhSyntaxin 12, and rhSyntaxin 16 is observed.

Clonality

Polyclonal

Host

Sheep

Isotype

IgG

Scientific Data Images for Human Syntaxin 6 Antibody

Detection of Human Syntaxin 6 by Western Blot.

Western blot shows lysates of human cortex tissue. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human Syntaxin 6 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5664) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Syntaxin 6 at approximately 35 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

Syntaxin 6 in Human Brain.

Syntaxin 6 was detected in immersion fixed paraffin-embedded sections of human brain (cortex) using Sheep Anti-Human Syntaxin 6 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5664) at 5 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counter-stained with hematoxylin (blue). Specific staining was localized to the cytoplasm of neurons. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Syntaxin 6 in HeLa Human Cell Line.

Syntaxin 6 was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line using Sheep Anti-Human Syntaxin 6 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5664) at 1.7 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 493-conjugated Anti-Sheep IgG Secondary Antibody (green; Catalog # NL012) and counterstained with DAPI (blue). Specific staining was localized to Golgi vesicles. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Applications for Human Syntaxin 6 Antibody

Application

Recommended Usage

Immunocytochemistry

5-15 µg/mL
Sample: Immersion fixed HeLa human cervical epithelial carcinoma cell line

Immunohistochemistry

5-15 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human brain (cortex)

Western Blot

1 µg/mL
Sample: Human cortex tissue

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Syntaxin 6

Syntaxin 6 (STX6) is a 30‑35 kDa member of the syntaxin family of proteins. It is widely expressed, embedded in trans‑Golgi and endosomal membranes, and is associated with a variety of SNARE proteins. STX6 is involved in the regulation of caveolae‑dependent endocytosis, and is crucial for membrane remodeling. Human STX6 is a type IV single‑pass transmembrane protein (very long cytoplasmic N‑terminus) that is 255 amino acids (aa) in length. It contains a coiled‑coil region (aa 41‑74), a t‑SNARE domain (aa 163‑225) that is likely involved in protein‑protein interactions, and a C‑terminal transmembrane sequence. There are two splice variants that are also 255 aa in length, but show variation over aa 69‑73. Over aa 69‑217, human STX6 shares 93% aa identity with mouse STX6.

Alternate Names

STX6

Entrez Gene IDs

10228 (Human); 58244 (Mouse); 60562 (Rat)

Gene Symbol