Human T-bet/TBX21 Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB5385
Conjugate
Catalog #
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human
Applications
Validated:
Immunocytochemistry, Intracellular Staining by Flow Cytometry, Western Blot
Cited:
Flow Cytometry, Immunohistochemistry, Immunohistochemistry-Paraffin
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # 525803
Product Specifications
Immunogen
E. coli-derived recombinant human T-bet
Glu326-Asn535
Accession # Q9UL17
Glu326-Asn535
Accession # Q9UL17
Specificity
Detects human T-bet.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Scientific Data Images for Human T-bet/TBX21 Antibody
Detection of T-bet/TBX21 in Jurkat Human Cell Line by Flow Cytometry.
Jurkat human acute T cell leukemia cell line was stained with Human T-bet/TBX21 Monoclonal Antibody (Catalog # MAB5385, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG F(ab')2Secondary Antibody (Catalog # F0102B). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with ice-cold methanol.
Detection of Human T-bet/TBX21 by Western Blot.
Western blot shows lysates of Raji human Burkitt's lymphoma cell line and Daudi human Burkitt's lymphoma cell line . PVDF membrane was probed with 2 µg/mL of Human T-bet/TBX21 Monoclonal Antibody (Catalog # MAB5385) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for T-bet/TBX21 at approximately 55 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.
Detection of T-bet/TBX21 in CD45RO+/CD8+ PBMC lymphocytes by Flow Cytometry
CD45RO+/CD8+ PBMC lymphocytes were stained with either (A) Mouse Anti-Human T-bet/TBX21 Monoclonal Antibody (Catalog # MAB5385) or (B) isotype control antibody (Catalog # MAB002) followed by Fluorescein-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0103B). Cells were then stained with Mouse Anti-Human CD8 alpha APC-conjugated Monoclonal Antibody (Catalog # FAB1509A) and Mouse Anti-Human CD45RO PE-conjugated Monoclonal Antibody (Catalog # FAB10642P). To facilitate intracellular staining, cells were fixed and permeabilized with FlowX FoxP3 Fixation & Permeabilization Buffer Kit (Catalog # FC012). View our protocol for Staining Intracellular Molecules.Applications for Human T-bet/TBX21 Antibody
Application
Recommended Usage
Immunocytochemistry
8-25 µg/mL
Sample: Immersion fixed Jurkat human acute T cell leukemia cell line
Sample: Immersion fixed Jurkat human acute T cell leukemia cell line
Intracellular Staining by Flow Cytometry
2.5 µg/106 cells
Sample: Jurkat human acute T cell leukemia cell line fixed with paraformaldehyde and permeabilized with ice-cold methanol
Sample: Jurkat human acute T cell leukemia cell line fixed with paraformaldehyde and permeabilized with ice-cold methanol
Western Blot
2 µg/mL
Sample: Raji human Burkitt's lymphoma cell line and Daudi human Burkitt's lymphoma cell line
Sample: Raji human Burkitt's lymphoma cell line and Daudi human Burkitt's lymphoma cell line
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Reviewed Applications
Read 1 review rated 5 using MAB5385 in the following applications:
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: T-bet/TBX21
T-box expressed in T cells (T-bet), also known as T-box transcription factor TBX21, is a 62 kDa member of the T-box family of transcription factors and the Tbr1 subfamily. Human T-bet is 535 amino acids in length and contains a T-box DNA binding domain (aa 136-327). Human T-bet shares 88% aa sequence identity with mouse T-bet. T-bet is a nuclear protein highly apparent in Th1-cells. Northern blot analysis revealed that it is also expressed in lung, thymus and spleen. Functionally, T-bet controls the expression of the Th1 cytokine, IFN gamma, and initiates Th1 lineage development from naïve Th precursor cells by both activating Th1 genetic programs and by repressing the opposing Th2 programs.
Long Name
T-box transcription factor TBX21
Alternate Names
T-PET, Tbet, TBLYM, TBX21
Gene Symbol
TBX21
UniProt
Additional T-bet/TBX21 Products
Product Documents for Human T-bet/TBX21 Antibody
Product Specific Notices for Human T-bet/TBX21 Antibody
For research use only
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