Human TACI/TNFRSF13B Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB1741
Clone 165604 was used by HLDA to establish CD designation
Conjugate
Catalog #
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human
Applications
Validated:
Blockade of Receptor-ligand Interaction, CyTOF-ready, Flow Cytometry
Cited:
Flow Cytometry
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # 165604
Product Specifications
Immunogen
Mouse myeloma cell line NS0-derived recombinant human TACI/TNFRSF13B
Ser2-Thr166
Accession # O14836
Ser2-Thr166
Accession # O14836
Specificity
Detects human TACI/TNFRSF13B in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human BCMA or recombinant mouse TACI is observed.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Scientific Data Images for Human TACI/TNFRSF13B Antibody
Detection of TACI/TNFRSF13B in Raji Human Cell Line by Flow Cytometry.
Raji human Burkitt's lymphoma cell line was stained with Mouse Anti-Human TACI/TNFRSF13B Monoclonal Antibody (Catalog # MAB1741, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram), followed by PerCP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0114).Applications for Human TACI/TNFRSF13B Antibody
Application
Recommended Usage
Blockade of Receptor-ligand Interaction
In a functional ELISA, 0.05-0.2 µg/mL of this antibody will block 50% of the binding of 50 ng/mL of Recombinant Human BAFF/BLyS/TNFSF13B (Catalog # 2149-BF) to immobilized Recombinant Human TACI/TNFRSF13B Fc Chimera (Catalog # 174-TC) coated at 1 µg/mL (100 µL/well). At 10 μg/mL, this antibody will block >90% of the binding.
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
Flow Cytometry
0.25 µg/106 cells
Sample: Raji human Burkitt's lymphoma cell line
Sample: Raji human Burkitt's lymphoma cell line
Reviewed Applications
Read 1 review rated 5 using MAB1741 in the following applications:
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: TACI/TNFRSF13B
TACI, transmembrane activator and CAML-interactor, is a member of the TNF receptor superfamily. TACI is a type III membrane protein with an extracellular N‑terminus in the absence of a cleaved signal sequence. The extracellular region of TACI contains two cysteine rich domains. Within the TNFRSF, it shares the highest homology with BCMA. TACI and BCMA have both been shown to bind APRIL and BAFF, members of the TNF ligand superfamily. TACI is expressed on the cell surface of B cells and activated, but not resting, T cells. Analogous to BCMA, data suggests that TACI may play an important role in B cell development, function and regulation. Human TACI is a 293 amino acid (aa) protein consisting of a 166 aa extracellular domain, a 20 aa transmembrane domain, and a 107 aa intracellular domain. Human and mouse TACI share 54% amino acid identity.
References
- Xia, X.-Z. et al. (2000) J. Exp. Med. 192:137.
- von Bulow, G.U. et al. (1997) Science, 278:138.
- Gross, J.A. et al. (2000) Nature 404:995.
- Marsters, S.A. et al. (2000) Curr. Biol. 10:785.
- Yan, M. et al. (2000) Nature Immunol. 1:37.
- Wu, Y. et al. (2000) J. Biol. Chem. 275:35478.
Long Name
Transmembrane Activator and CAML Interactor/Tumor Necrosis Factor Receptor Superfamily Member 13B
Alternate Names
CD267, CVID, TNFRSF13B
Gene Symbol
TNFRSF13B
UniProt
Additional TACI/TNFRSF13B Products
Product Documents for Human TACI/TNFRSF13B Antibody
Product Specific Notices for Human TACI/TNFRSF13B Antibody
For research use only
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