Human TACI/TNFRSF13B Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB174
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human
Applications
Validated:
Blockade of Receptor-ligand Interaction, ELISA Capture (Matched Antibody Pair), Immunohistochemistry, Western Blot
Cited:
Flow Cytometry, Immunocytochemistry, Immunoprecipitation, Neutralization, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # 165609
Product Specifications
Immunogen
Mouse myeloma cell line NS0-derived recombinant human TACI/TNFRSF13B
Ser2-Thr166
Accession # O14836
Ser2-Thr166
Accession # O14836
Specificity
Detects human TACI/TNFRSF13B in ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant mouse TACI or recombinant human BCMA is observed.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Scientific Data Images for Human TACI/TNFRSF13B Antibody
Detection of Human TACI/TNFRSF13B/CVID by Immunocytochemistry/Immunofluorescence
Expressions of BLyS, TACI, BCMA and BAFF-R in human breast cancer cell lines. (A) BLyS and its three receptors in human breast cancer cell lines MDA-MB-435, MDA-MB-231, MDA-MB-468 and B cell line Ramos by immunofluorescence (original magnification 200 ×) and Western Blotting. (B) The mRNA level of BLyS in the three cell lines were detected by real-time PCR under hypoxia for different time points. Data were means of triplicate samples with ± SD; vs normoxia, *, P < 0.05; **, P < 0.01; ***, P < 0.001. (C) BLyS protein level in MDA-MB-435 cells by Western Blotting analysis. Image collected and cropped by CiteAb from the following publication (https://jeccr.biomedcentral.com/articles/10.1186/1756-9966-31-31), licensed under a CC-BY license. Not internally tested by R&D Systems.Applications for Human TACI/TNFRSF13B Antibody
Application
Recommended Usage
Blockade of Receptor-ligand Interaction
In a functional ELISA, 0.03-0.12 µg/mL of this antibody will block 50% of the binding of 50 ng/mL of Recombinant Human BAFF/BLyS/TNFSF13B (Catalog # 2149-BF) to immobilized Recombinant Human TACI/TNFRSF13B Fc Chimera (Catalog # 174-TC) coated at 1 µg/mL (100 µL/well) . At 10 μg/mL, this antibody will block >90% of the binding.
Immunohistochemistry
8-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human spleen
Sample: Immersion fixed paraffin-embedded sections of human spleen
Western Blot
1 µg/mL
Sample: Recombinant Human TACI/TNFRSF13B Fc Chimera (Catalog # 174-TC)
Sample: Recombinant Human TACI/TNFRSF13B Fc Chimera (Catalog # 174-TC)
Human TACI/TNFRSF13B Sandwich Immunoassay
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: TACI/TNFRSF13B
TACI, transmembrane activator and CAML-interactor, is a member of the TNF receptor superfamily. TACI is a type III membrane protein with an extracellular N‑terminus in the absence of a cleaved signal sequence. The extracellular region of TACI contains two cysteine rich domains. Within the TNFRSF, it shares the highest homology with BCMA. TACI and BCMA have both been shown to bind APRIL and BAFF, members of the TNF ligand superfamily. TACI is expressed on the cell surface of B cells and activated, but not resting, T cells. Analogous to BCMA, data suggests that TACI may play an important role in B cell development, function and regulation. Human TACI is a 293 amino acid (aa) protein consisting of a 166 aa extracellular domain, a 20 aa transmembrane domain, and a 107 aa intracellular domain. Human and mouse TACI share 54% amino acid identity.
References
- Xia, X.-Z. et al. (2000) J. Exp. Med. 192:137.
- von Bulow, G.U. et al. (1997) Science, 278:138.
- Gross, J.A. et al. (2000) Nature 404:995.
- Marsters, S.A. et al. (2000) Curr. Biol. 10:785.
- Yan, M. et al. (2000) Nature Immunol. 1:37.
- Wu, Y. et al. (2000) J. Biol. Chem. 275:35478.
Long Name
Transmembrane Activator and CAML Interactor/Tumor Necrosis Factor Receptor Superfamily Member 13B
Alternate Names
CD267, CVID, TNFRSF13B
Gene Symbol
TNFRSF13B
UniProt
Additional TACI/TNFRSF13B Products
Product Documents for Human TACI/TNFRSF13B Antibody
Product Specific Notices for Human TACI/TNFRSF13B Antibody
For research use only
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