Human TAFA3/FAM19A3 Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB4677
Conjugate
Catalog #
Key Product Details
Species Reactivity
Human
Applications
CyTOF-ready, Intracellular Staining by Flow Cytometry, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2B Clone # 460904
Product Specifications
Immunogen
E. coli-derived recombinant human TAFA3/FAM19A3
Ala31-Arg133
Accession # Q7Z5A8
Ala31-Arg133
Accession # Q7Z5A8
Specificity
Detects human TAFA3/FAM19A3 in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross‑reactivity with recombinant human TAFA1, 2, 4, or 5 is observed.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2B
Scientific Data Images for Human TAFA3/FAM19A3 Antibody
Detection of TAFA3/FAM19A3 in A172 Human Cell Line by Flow Cytometry.
A172 human glioblastoma cell line was stained with Mouse Anti-Human TAFA3/FAM19A3 Monoclonal Antibody (Catalog # MAB4677, filled histogram) or isotype control antibody (Catalog # MAB0041, open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG F(ab')2Secondary Antibody (Catalog # F0101B). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with saponin.Applications for Human TAFA3/FAM19A3 Antibody
Application
Recommended Usage
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
Intracellular Staining by Flow Cytometry
2.5 µg/106 cells
Sample: A172 human glioblastoma cell line fixed with paraformaldehyde and permeabilized with saponin
Sample: A172 human glioblastoma cell line fixed with paraformaldehyde and permeabilized with saponin
Western Blot
1 µg/mL
Sample: Recombinant Human TAFA3/FAM19A3 under non-reducing conditions only
Sample: Recombinant Human TAFA3/FAM19A3 under non-reducing conditions only
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: TAFA3/FAM19A3
TAFA3 (also FAM19A3) is an 11 kDa (predicted) member of the FAM19/TAFA family of 5 proteins that show differential expression in the brain. It is probably a secreted protein. TAFA 1‑4 proteins bear chemokine-like signatures, and are distantly related to MIP-1 alpha, sharing its CC motif. A conserved 10 cysteine pattern suggests multiple disulfide linkages. Mature human TAFA3 is 103 aa in length and has no predicted N‑linked glycosylation sites. It shares 86% aa identity with mouse TAFA3. A potential 131 aa isoform diverges at aa 59 of the mature sequence.
Long Name
Family with Sequence Similarity 19, Member A3
Alternate Names
FAM19A3
Gene Symbol
TAFA3
UniProt
Additional TAFA3/FAM19A3 Products
Product Documents for Human TAFA3/FAM19A3 Antibody
Product Specific Notices for Human TAFA3/FAM19A3 Antibody
For research use only
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