Human TCF-2/HNF-1 beta Antibody
R&D Systems, part of Bio-Techne | Catalog # AF3330
Key Product Details
Species Reactivity
Human
Applications
Immunocytochemistry, Immunohistochemistry
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Product Specifications
Immunogen
E. coli-derived recombinant human TCF‑2/HNF‑1 beta
Gly51-Gln182
Accession # P35680
Gly51-Gln182
Accession # P35680
Specificity
This antibody detects TCF‑2/HNF‑1 beta in direct ELISAs and Western blots. It is expected to recognize all three TCF‑2 isoforms.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for Human TCF-2/HNF-1 beta Antibody
TCF-2/HNF-1 beta in BG01V Human Embryonic Stem Cells.
TCF-2/HNF-1 beta was detected in immersion fixed BG01V human embryonic stem cells differentiated into pancreatic progenitor cells using Goat Anti-Human TCF-2/HNF-1 beta Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3330) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Stem Cells on Coverslips.
TCF‑2/HNF‑1 beta in Human Kidney.
TCF-2/HNF-1 beta was detected in immersion fixed paraffin-embedded sections of human kidney using Goat Anti-Human TCF-2/HNF-1 beta Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3330) at 3 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to nuclei in convoluted tubules. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
TCF‑2/HNF‑1 beta in Rat Kidney.
TCF-2/HNF-1 beta was detected in perfusion fixed frozen sections of rat kidney using Goat Anti-Human TCF-2/HNF-1 beta Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3330) at 3 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to nuclei in convoluted tubules. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.Applications for Human TCF-2/HNF-1 beta Antibody
Application
Recommended Usage
Immunocytochemistry
5-15 µg/mL
Sample: Immersion fixed HepG2 human hepatocellular carcinoma cell line and BG01V human embryonic stem cells differentiated into pancreatic progenitor cells
Sample: Immersion fixed HepG2 human hepatocellular carcinoma cell line and BG01V human embryonic stem cells differentiated into pancreatic progenitor cells
Immunohistochemistry
3-15 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human kidney and perfusion fixed frozen sections of rat kidney
Sample: Immersion fixed paraffin-embedded sections of human kidney and perfusion fixed frozen sections of rat kidney
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: TCF-2/HNF-1 beta
Long Name
Transcription Factor 2
Alternate Names
HNF-1 beta, HNF1B, HNF2, LF-B3, MODY5, TCF2, VHNF1
Gene Symbol
HNF1B
UniProt
Additional TCF-2/HNF-1 beta Products
Product Documents for Human TCF-2/HNF-1 beta Antibody
Product Specific Notices for Human TCF-2/HNF-1 beta Antibody
For research use only
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