Human TEM7/PLXDC1 Isoform 1 Antibody
R&D Systems, part of Bio-Techne | Catalog # AF5327
Key Product Details
Species Reactivity
Human
Applications
Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Sheep IgG
Product Specifications
Immunogen
Mouse myeloma cell line NS0-derived recombinant human TEM7/PLXDC1 Isoform 1
Leu19-Thr426
Accession # ACE86780
Leu19-Thr426
Accession # ACE86780
Specificity
Detects human TEM7/PLXDC1 Isoform 1 in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant human TEM8 is observed.
Clonality
Polyclonal
Host
Sheep
Isotype
IgG
Scientific Data Images for Human TEM7/PLXDC1 Isoform 1 Antibody
Detection of Human TEM7/PLXDC1 Isoform 1 by Western Blot.
Western blot shows lysates of HT-29 human colon adenocarcinoma cell line cancer tissue, HeLa human cervical epithelial carcinoma cell line, and MCF-7 human breast cancer cell line. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human TEM7/PLXDC1 Isoform 1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5327) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for TEM7 Isoform 1 at approximately 85 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.Applications for Human TEM7/PLXDC1 Isoform 1 Antibody
Application
Recommended Usage
Western Blot
1 µg/mL
Sample: HT-29 human colon adenocarcinoma cell line cancer tissue, HeLa human cervical epithelial carcinoma cell line, and MCF-7 human breast cancer cell line
Sample: HT-29 human colon adenocarcinoma cell line cancer tissue, HeLa human cervical epithelial carcinoma cell line, and MCF-7 human breast cancer cell line
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: TEM7/PLXDC1
TEM7 (Tumor endothelial marker 7; also TEM3 and Plexin domain-containing protein-1/PXDC1) is an 85 kDa member of the plexin family of proteins. It is one of a number of TEM designated molecules that are related only a name and not structural similarity. TEM7 is found on tumor endothelial cells, newly formed endothelial cells and neurons. It is known to bind nidogen. Mature human TEM7 is a type I transmembrane glycoprotein 482 amino acids (aa) in length. It contains an extracellular region (aa 19‑426) with one plexin domain (aa 303‑347). There are three isoform variants. One is intracellular and shows an alternate start site at Met74 accompanied by a 28 aa substitution for aa 331‑500. The other two are soluble, with one showing only the above 28 aa substitution and a second showing an eight aa substitution for aa 331‑500. Over aa 19‑426, human TEM7 shares 81% aa identity with mouse TEM7.
Long Name
Tumor Endothelial Marker 7/Plexin Domain Containing Protein 1
Alternate Names
PLXDC1, TEM3
Gene Symbol
PLXDC1
UniProt
Additional TEM7/PLXDC1 Products
Product Documents for Human TEM7/PLXDC1 Isoform 1 Antibody
Product Specific Notices for Human TEM7/PLXDC1 Isoform 1 Antibody
For research use only
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