Human TEX19 Antibody
R&D Systems, part of Bio-Techne | Catalog # AF6319
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human
Applications
Validated:
Immunocytochemistry, Western Blot
Cited:
Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Sheep IgG
Product Specifications
Immunogen
E. coli-derived recombinant human TEX19
Met1-Ser164
Accession # Q8NA77
Met1-Ser164
Accession # Q8NA77
Specificity
Detects human TEX19 in direct ELISAs and Western blots. In direct ELISAs, less than 2% cross-reactivity with recombinant human TEX11 is observed.
Clonality
Polyclonal
Host
Sheep
Isotype
IgG
Scientific Data Images for Human TEX19 Antibody
Detection of Human TEX19 by Western Blot.
Western blot shows lysates of NTera-2 human testicular embryonic carcinoma cell line. PVDF Membrane was probed with 0.5 µg/mL of Human TEX19 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6319) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for TEX19 at approximately 18 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.
TEX19 in BG01V Human Stem Cells.
TEX19 was detected in immersion fixed BG01V human embryonic stem cells using Human TEX19 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6319) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red, upper panel; Catalog # NL010) and counterstained with DAPI (blue, lower panel). Specific staining was localized to nuclei and cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.Applications for Human TEX19 Antibody
Application
Recommended Usage
Immunocytochemistry
10 µg/mL
Sample: Immersion fixed BG01V human embryonic stem cells
Sample: Immersion fixed BG01V human embryonic stem cells
Western Blot
0.5 µg/mL
Sample: NTera‑2 human testicular embryonic carcinoma cell line
Sample: NTera‑2 human testicular embryonic carcinoma cell line
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Sterile PBS to a final concentration of 0.2 mg/mL. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: TEX19
TEX19 (testis expressed sequence 19) is an 18 kDa (predicted) protein with restricted cell expression. It is expressed in spermatogonia as well as pluripotent stem cells. TEX19 has been found in both nucleus and cytoplasm. Functionally, TEX19 is proposed to guard against inappropriately activated endogenous retroviruses during meiosis, and thus prevent deleterious mutations. Human TEX19 is 164 amino acids (aa) in length. There is no identifiable structural module within the protein. There are, however, two sequences that are conserved in TEX19 from multiple species. These are an MCP domain between aa 1-58, and a VPTEL domain that spans aa 120-157. Over aa 1-164, human TEX19 shares only 53% aa identity with a highly divergent and substantially larger 42 kDa mouse TEX19.1 protein.
Long Name
Testis Expressed 19
Alternate Names
FLJ35767
Gene Symbol
TEX19
UniProt
Additional TEX19 Products
Product Documents for Human TEX19 Antibody
Product Specific Notices for Human TEX19 Antibody
For research use only
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