Key Product Details

Species Reactivity

Human

Applications

CyTOF-ready, Intracellular Staining by Flow Cytometry

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # 701010

View all Themis Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human Themis
Ala2-Pro282
Accession # Q8N1K5

Specificity

Detects human Themis in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant mouse Themis is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Scientific Data Images for Human Themis Antibody

Detection of Themis antibody in Human Blood Lymphocytes antibody by Flow Cytometry

Detection of Themis in Human Blood Lymphocytes by Flow Cytometry

Human peripheral blood lymphocytes were stained with Mouse Anti-Human Themis Monoclonal Antibody (Catalog # MAB6929) followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B) and Mouse Anti-Human CD3e APC-conjugated Monoclonal Antibody (Catalog # FAB100A). Quadrant markers were set based on control antibody staining (Catalog # MAB003).

Applications for Human Themis Antibody

Application
Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Intracellular Staining by Flow Cytometry

2.5 µg/106 cells
Sample: Human peripheral blood lymphocytes fixed with paraformaldehyde and permeabilized with saponin.

 

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.

Reconstitution Buffer Available:
Size / Price
Qty

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Themis

THEMIS (thymocyte-expressed molecule involved in selection), also called Gasp (Grb2-associating protein), is a 72 kDa, 641 amino acid (aa) cytosolic phosphoprotein mainly expressed in late double-negative and double-positive thymocytes. It is involved in thymocyte positive and negative selection through regulation of TCR signaling. Within the region used as an immunogen, human THEMIS shares 86% aa sequence identity with mouse and rat THEMIS.

Long Name

Thymocyte-expressed Molecule Involved in Selection

Alternate Names

C6orf190, C6orf207, Gasp, SPOT, TSEPA

Entrez Gene IDs

387357 (Human); 210757 (Mouse)

Gene Symbol

THEMIS

UniProt

Q8N1K5 (Human)

Additional Themis Products

Product Documents for Human Themis Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Human Themis Antibody

For research use only

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