Human TIMP-4 Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB974
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human
Applications
Validated:
ELISA Capture (Matched Antibody Pair), Immunohistochemistry, Western Blot
Cited:
Immunocytochemistry, Immunohistochemistry-Frozen, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # 153934
Product Specifications
Immunogen
Mouse myeloma cell line NS0-derived recombinant human TIMP-4
Cys30-Pro244
Accession # Q99727
Cys30-Pro244
Accession # Q99727
Specificity
Detects human TIMP-4 in ELISAs and Western blots. In ELISAs, no cross-reactivity with recombinant human TIMP-1, -2, -3, or recombinant mouse TIMP-1 is observed.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Scientific Data Images for Human TIMP-4 Antibody
TIMP-4 in Human Breast Cancer Tissue.
TIMP-4 was detected in immersion fixed paraffin-embedded sections of human breast cancer tissue using 25 µg/mL Mouse Anti-Human TIMP-4 Monoclonal Antibody (Catalog # MAB974) overnight at 4 °C. Tissue was stained with the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.Applications for Human TIMP-4 Antibody
Application
Recommended Usage
Immunohistochemistry
8-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human breast cancer tissue
Sample: Immersion fixed paraffin-embedded sections of human breast cancer tissue
Western Blot
1 µg/mL
Sample: Recombinant Human TIMP-4 (Catalog # 974-TSF)
Sample: Recombinant Human TIMP-4 (Catalog # 974-TSF)
Human TIMP-4 Sandwich Immunoassay
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: TIMP-4
Long Name
Tissue Inhibitors of Metalloproteinases 4
Alternate Names
TIMP4
Gene Symbol
TIMP4
UniProt
Additional TIMP-4 Products
Product Documents for Human TIMP-4 Antibody
Product Specific Notices for Human TIMP-4 Antibody
For research use only
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