Human Tissue alpha-L-Fucosidase/FUCA1 Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB7039

R&D Systems, part of Bio-Techne
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MAB7039
MAB7039-SP
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Key Product Details

Species Reactivity

Human

Applications

Simple Western, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 810607

View all Tissue alpha-L-Fucosidase/FUCA1 Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant human Tissue alpha‑L‑Fucosidase/FUCA1
Gln32-Lys466
Accession # P04066

Specificity

Detects human Tissue alpha‑L‑Fucosidase/FUCA1 in direct ELISAs and Western blots.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Human Tissue alpha-L-Fucosidase/FUCA1 Antibody

Detection of Human Tissue a-L-Fucosidase/FUCA1 antibody by Western Blot.

Detection of Human Tissue alpha‑L‑Fucosidase/FUCA1 by Western Blot.

Western blot shows lysates of human placenta tissue and human thyroid tissue. PVDF membrane was probed with 0.2 µg/mL of Mouse Anti-Human Tissue a-L-Fucosidase/FUCA1 Monoclonal Antibody (Catalog # MAB7039) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for Tissue a-L-Fucosidase/FUCA1 at approximately 50 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Detection of Human Tissue a-L-Fucosidase/FUCA1 antibody by Simple WesternTM.

Detection of Human Tissue alpha‑L‑Fucosidase/FUCA1 by Simple WesternTM.

Simple Western lane view shows lysates of human thyroid tissue, loaded at 0.5 mg/mL. A specific band was detected for Tissue alpha‑L‑Fucosidase/FUCA1 at approximately 56 kDa (as indicated) using 2 µg/mL of Mouse Anti-Human Tissue alpha‑L‑Fucosidase/FUCA1 Monoclonal Antibody (Catalog # MAB7039). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Applications for Human Tissue alpha-L-Fucosidase/FUCA1 Antibody

Application
Recommended Usage

Simple Western

2 µg/mL
Sample: Human thyroid tissue

Western Blot

0.2 µg/mL
Sample: Human placenta tissue and human thyroid tissue
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.

Reconstitution Buffer Available:
Size / Price
Qty

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Tissue alpha-L-Fucosidase/FUCA1

Fucosylated glycoconjugates play numerous roles in biological processes, such as development, apoptosis and the immune responses, and are involved in the pathology of inflammation, cancer and cystic fibrosis (1, 2, 3, 4). Tissue alpha‑L‑Fucosidase, also known as FUCA1, is a lysosomal enzyme responsible for hydrolyzing alpha‑L‑fucose moieties from glycolipids and oligosaccharides (5). The mature enzyme in the liver has a molecular weight of 200 kDa and exists in the native state as a tetramer (6). Defects in FUCA1 are the cause of fucosidosis (7, 8, 9), an autosomal recessive lysosomal storage disease characterized by the accumulation of fucose-containing glycolipids and glycoproteins in various tissues. Serum alpha‑L‑fucosidase has been identified as a useful marker for oral cancer (10).

References

  1. Hiraishi, K. et al. (1993) Glycobiology 3:381.
  2. Solter, D. and Knowles, B.B. (1978) Proc. Natl. Acad. Sci. USA 75:5565.
  3. Becker, D.J. and Lowe, J.B. (2003) Glycobiology 13:41R.
  4. Liu, T.W. et al. (2009) Proc. Natl. Acad. Sci. USA 106:14581.
  5. Fukushima, H. et al. (1985) Proc. Natl. Acad. Sci. USA 82:1262.
  6. Alhadeff, J.A. et al. (1975) J. Biol. Chem. 250:7106.
  7. O’Brien, J.S. et al. (1987) Enzyme 38:45.
  8. Durand, P. et al. (1969) J. Pediatr. 75:665.
  9. Johnson, K. and Dawson, G. (1985) Biochem. Biophys. Res. Commun. 133:90.
  10. Shah, M. et al. (2008) Cancer 113:336.

Alternate Names

FUCA1, Tissue alphaLFucosidase

Entrez Gene IDs

2517 (Human); 71665 (Mouse); 24375 (Rat)

Gene Symbol

FUCA1

UniProt

P04066

Additional Tissue alpha-L-Fucosidase/FUCA1 Products

Product Documents for Human Tissue alpha-L-Fucosidase/FUCA1 Antibody

Product Datasheets

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human Tissue alpha-L-Fucosidase/FUCA1 Antibody

For research use only

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