Human TLE1 Antibody

R&D Systems, part of Bio-Techne | Catalog # AF5947

R&D Systems, part of Bio-Techne
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AF5947
AF5947-SP
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Key Product Details

Species Reactivity

Validated:

Human

Cited:

Mouse

Applications

Validated:

Western Blot

Cited:

Immunohistochemistry

Label

Unconjugated

Antibody Source

Polyclonal Sheep IgG

View all TLE1 Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human TLE1
His11-Asn200
Accession # Q04724

Specificity

Detects human TLE1 in direct ELISAs and Western blots. In direct ELISAs, approximately 10% cross-reactivity with recombinant human (rh) TLE3 and rhTLE4 is observed and less than 5% cross-reactivity with rhTLE2 is observed.

Clonality

Polyclonal

Host

Sheep

Isotype

IgG

Scientific Data Images for Human TLE1 Antibody

Detection of Human TLE1 antibody by Western Blot.

Detection of Human TLE1 by Western Blot.

Western blot shows lysates of human muscle tissue and human heart tissue. PVDF Membrane was probed with 2 µg/mL of Human TLE1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5947) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for TLE1 at approximately 100 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

Applications for Human TLE1 Antibody

Application
Recommended Usage

Western Blot

2 µg/mL
Sample: Human muscle tissue and human heart tissue

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

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Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: TLE1

TLE1 (Transducin-Like Enhancer of Split 1; also ESG1 and GRG1) is a member of the WD repeat Groucho/TLE family of transcriptional repressors. It is widely expressed, and is known to antagonize NFkB and TCF (Wnt)-mediated signaling. TLE1 binds to other family members as a heterooligomer, or to itself as a homooligomer. While it possesses no intrinsic DNA-binding activity, it does modulate the activity of bound transcription factors. Human TLE1 is 770 amino acids (aa) in length. It contains a Gln‑rich region that mediates oligomerization (aa 1-131), a CCN domain that contains an NLS and CDC2 kinase site (aa 200-268), and six WD repeats that mediate protein‑protein interaction (aa 470-767). Phosphorylation on Ser will generate 90-93 kDa and 116 kDa forms in SDS‑Page. Potential splice variants exist. There are alternative start sites at Met57 and Met326, a 10 aa insertion after Gly124, and three distinct eight aa substitutions for aa 193-770, 193-199 and 445-770. Over aa 11-200, human TLE1 shares 98% aa identity with mouse TLE1.

Long Name

Transducin-like Enhancer of Split 1

Alternate Names

GRG1

Entrez Gene IDs

7088 (Human); 21885 (Mouse); 362533 (Rat)

Gene Symbol

TLE1

UniProt

Q04724 (Human)

Additional TLE1 Products

Product Documents for Human TLE1 Antibody

Product Datasheets

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human TLE1 Antibody

For research use only

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