HEK293 human embryonic kidney cell line transfected with human TMIGD2/CD28H and eGFP was stained with and either (A) Mouse Anti-Human TMIGD2/CD28H PE-conjugated Monoclonal Antibody (Catalog # FAB83162P) or (B) Mouse IgG2A Isotype Control (Catalog # IC003P). View our protocol for Staining Membrane-associated Proteins.

Applications for Human TMIGD2/CD28H PE-conjugated Antibody

Application

Recommended Usage

Flow Cytometry

10 µL/10⁶ cells
Sample: HEK293 Human Cell Line Transfected with Human TMIGD2/CD28H and eGFP

Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.

12 months from date of receipt, 2 to 8 °C as supplied.

Background: TMIGD2/CD28H

CD28 homolog (CD28H), also called TMIG2 and IGPR-1, is a 55 kDa glycosylated transmembrane protein that shares approximately 10% amino acid (aa) sequence identity with CD28, CTLA-4, ICOS, and PD-1. CD28H is composed of a single extracellular immunoglobulin variable-like domain (IgV) (aa 23-109), a transmembrane domain (aa 151-171), and a long cytoplasmic domain (aa172-282). CD28H is constitutively expressed on naive T and NK cells. Similar to the interaction of B7 with CD28, the interaction of CD28H with B7-H7 activates the Akt-dependent signaling cascade and promotes the proliferation and activation of newly generated peripheral effector and memory T cells (1, 2). CD28H is additionally expressed in the skin and epithelium lining the lung, airway, mammary gland, and gastrointestinal tract (3). It regulates cellular morphology, focal adhesion contact formation, and cell migration (3, 4). CD28H also participates in angiogenesis in vitro (3). CD28H interacts with multiple cytoskeletal proteins including Actin, Paxillin, SPIN90, CACNB2, and BPAG1. Interactions between the cytoplasmic proline-rich domain of CD28H and SPIN90 modulate the activity of CD28H in both angiogenesis and cell adhesion (3, 5).

References

Zhu, Y. et al. (2013) Nat. Commun. 4:2043.
Parry, R.V. et al. (2003) J.Immunol. 171:166.
Rahimi, N. et al. (2012) Mol. Biol. Cell. 23:1646.
Huang, C. et al. (2003) Nature 424:219.
Kaneko, T. et al. (2008) Front. Biosci. 13:4938.

Long Name

Transmembrane And Immunoglobulin Domain Containing 2

Alternate Names

CD28H, IGPR-1, IGPR1

Entrez Gene IDs

126259 (Human)

Gene Symbol

TMIGD2

UniProt

Q96BF3

Additional TMIGD2/CD28H Products

Product Documents for Human TMIGD2/CD28H PE-conjugated Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Human TMIGD2/CD28H PE-conjugated Antibody

For research use only

Citations
Reviews

Spectra Viewer
Flow Cytometry

Human TMIGD2/CD28H PE-conjugated Antibody

R&D Systems, part of Bio-Techne | Catalog # FAB83162P

Key Product Details

Species Reactivity

Applications

Label

Antibody Source

View all TMIGD2/CD28H Antibodies »

Product Specifications

Immunogen

Specificity

Clonality

Host

Isotype

Scientific Data Images for Human TMIGD2/CD28H PE-conjugated Antibody

Detection of TMIGD2/CD28H in HEK293 Human Cell Line Transfected with Human TMIGD2/CD28H and eGFP by Flow Cytometry.