Human TNF RI/TNFRSF1A Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB225R
Recombinant Monoclonal Antibody.
Conjugate
Catalog #
Key Product Details
Species Reactivity
Human
Applications
Flow Cytometry, Neutralization
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Mouse IgG1 Clone # 16803R
Product Specifications
Immunogen
E. coli-derived recombinant human TNF RI/TNFRSF1A
Accession # NP_001056
Accession # NP_001056
Specificity
Detects human TNF R1 in Western blots. In Western blots, no cross-reactivity with recombinant human TNF RII, recombinant mouse (rm) TNF RI, or rmTNF RII is observed.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Scientific Data Images for Human TNF RI/TNFRSF1A Antibody
Detection of TNF RI/TNFRSF1A in Human Blood Monocytes by Flow Cytometry.
Human peripheral blood monocytes were stained with Recombinant Mouse Anti-Human TNF RI/TNFRSF1A Monoclonal Antibody (Catalog # MAB225R, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram), followed by APC-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B).
TNF RI/TNFRSF1A Inhibition of TNF-alpha induced Cytotoxicity and Neutralization by Human TNF RI/TNFRSF1A Antibody.
Recombinant Human TNF RI/TNFRSF1A (Catalog # 372-R1) inhibits Recombinant Human TNF-a (Catalog # 210-TA) induced cytotoxicity in the L-929 mouse fibroblast cell line in a dose-dependent manner (orange line), as measured by Resazurin (Catalog # AR002). Inhibition of Recombinant Human TNF-a (0.25 ng/mL) activity elicited by Recombinant Human TNF RI/TNFRSF1A (15 ng/mL) is neutralized (green line) by increasing concentrations of Human TNF RI/TNFRSF1A Recombinant Monoclonal Antibody (Catalog # MAB225R). The ND50 is typically 0.008-0.04 µg/mL in the presence of the metabolic inhibitor actinomycin D (1 µg/mL).Applications for Human TNF RI/TNFRSF1A Antibody
Application
Recommended Usage
Flow Cytometry
0.25 µg/106 cells
Sample: Human peripheral blood monocytes
Sample: Human peripheral blood monocytes
Neutralization
Measured by its ability to neutralize TNF RI/TNFRSF1A-mediated inhibition of cytotoxicity in the L-929 mouse fibroblast cell line. The Neutralization Dose (ND50) is typically 0.008-0.04 μg/mL in the presence of 15 ng/mL Recombinant Human TNF RI/TNFRSF1A, 0.25 ng/mL Recombinant Human TNF-alpha, and 1 μg/mL actinomycin D.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5
mg/mL in sterile PBS.
For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: TNF RI/TNFRSF1A
Long Name
Tumor Necrosis Factor Receptor I
Alternate Names
CD120a, TNFRI, TNFRSF1A
Gene Symbol
TNFRSF1A
UniProt
Additional TNF RI/TNFRSF1A Products
Product Documents for Human TNF RI/TNFRSF1A Antibody
Product Specific Notices for Human TNF RI/TNFRSF1A Antibody
For research use only
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