Human TRA-1-60(R) PE-conjugated Antibody
R&D Systems, part of Bio-Techne | Catalog # FAB4770P
Conjugate
Catalog #
Key Product Details
Species Reactivity
Human
Applications
Flow Cytometry
Label
Phycoerythrin (Excitation = 488 nm, Emission = 565-605 nm)
Antibody Source
Monoclonal Mouse IgM Clone # TRA-1-60
Product Specifications
Immunogen
2102Ep human embryonal carcinoma cell line
Specificity
Detects human TRA‑1‑60(R).
Clonality
Monoclonal
Host
Mouse
Isotype
IgM
Scientific Data Images for Human TRA-1-60(R) PE-conjugated Antibody
Detection of TRA‑1‑60(R) in BG01V Human Cells by Flow Cytometry.
BG01V human embryonic stem cells were stained with Mouse Anti-Human TRA-1-60(R) PE-conjugated Monoclonal Antibody (Catalog # FAB4770P, filled histogram) or isotype control antibody (Catalog # IC015P, open histogram). View our protocol for Staining Membrane-associated Proteins.Applications for Human TRA-1-60(R) PE-conjugated Antibody
Application
Recommended Usage
Flow Cytometry
10 µL/106 cells
Sample: BGO1V Human Embryonic Stem Cells
Sample: BGO1V Human Embryonic Stem Cells
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Reviewed Applications
Read 1 review rated 5 using FAB4770P in the following applications:
Formulation, Preparation, and Storage
Purification
IgM-specific Affinity-purified from hybridoma culture supernatant
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
- 12 months from date of receipt, 2 to 8 °C as supplied.
Background: TRA-1-60(R)
TRA-1-60 is a monoclonal antibody raised against a cell surface antigen of human embryonal carcinoma (EC) cells (1). The TRA-1-60 epitope is also found on human embryonic stem (ES) cells and primordial germ cells, and TRA-1-60 serves as a serum marker in patients with germ cell tumors (2-4). Investigation into the identity of the TRA-1-60 epitope demonstrated that it is a carbohydrate carried by a cell surface, sialylated, keratan sulfate proteoglycan (5). Subsequent evidence implicated podocalyxin as a carrier for the TRA-1-60 epitope (6).
References
- Andrews, P. et al. (1984) Hybridoma 3:347.
- Thomson, J. et al. (1998) Science 282:1145.
- Giwercman, A. et al. (1993) Cancer 72:1308.
- Marrink, J. et al. (1991) Int. J. Cancer 49:368.
- Badcock, G. et al. (1999) Cancer Res. 59:4715.
- Schopperle, W. and W. DeWolf (2007) Stem Cells 25:723.
Long Name
Tumor-related Antigen-1-60 [R]
Alternate Names
TRA160(R)
Entrez Gene IDs
5420 (Human)
Gene Symbol
PODXL
Additional TRA-1-60(R) Products
Product Documents for Human TRA-1-60(R) PE-conjugated Antibody
Product Specific Notices for Human TRA-1-60(R) PE-conjugated Antibody
For research use only
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