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Human TRA-1-81 PE-conjugated Antibody

R&D Systems, part of Bio-Techne | Catalog # FAB8495P

R&D Systems, part of Bio-Techne

Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Flow Cytometry

Cited:

Flow Cytometry

Label

Phycoerythrin (Excitation = 488 nm, Emission = 565-605 nm)

Antibody Source

Monoclonal Mouse IgM Clone # TRA-1-81

Product Specifications

Immunogen

Human embryonal carcinoma cell line 2102Ep

Specificity

Detects human TRA-1-81 in flow cytometry.

Clonality

Monoclonal

Host

Mouse

Isotype

IgM

Scientific Data Images for Human TRA-1-81 PE-conjugated Antibody

Detection of TRA-1-81 antibody in BG01V Human Cells antibody by Flow Cytometry.

Detection of TRA-1-81 in BG01V Human Cells by Flow Cytometry.

BG01V human embryonic stem cells were stained with Mouse Anti-Human TRA-1-81 PE-conjugated Monoclonal Antibody (Catalog # FAB8495P, filled histogram) or isotype control antibody (Catalog # IC015P, open histogram). View our protocol for Staining Membrane-associated Proteins.

Detection of TRA-1-81 in iPSC cells by Flow Cytometry.

iPSC cells were stained with Mouse Anti-Human TRA-1-81 PE-conjugated Monoclonal Antibody (Catalog # FAB8495P, filled histogram) or isotype control antibody (Catalog # IC015P, open histogram). View our protocol for Staining Membrane-associated Proteins.

Applications for Human TRA-1-81 PE-conjugated Antibody

Application
Recommended Usage

Flow Cytometry

10 µL/106 cells
Sample: BG01V human embryonic stem cells, iPSC cells

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: TRA-1-81

The TRA-1-81 antibody reacts with a high-molecular-mass carbohydrate epitope on the surface of human embryonal carcinoma (EC), embryonic germ (EG), embryonic stem (ES), and induced pluripotent stem (iPS) cells.  TRA-1-81 is an epitope expressed on podocalyxin, also known as Podocalyxin-Like Protein-1 (PCLP1 or PODXL), a type I transmembrane glycoprotein.

Long Name

Tumor-related Antigen-1-81

Alternate Names

TRA181

Entrez Gene IDs

5420 (Human)

Gene Symbol

PODXL

Additional TRA-1-81 Products

Product Documents for Human TRA-1-81 PE-conjugated Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Human TRA-1-81 PE-conjugated Antibody

For research use only

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