Human TRACP/PAP/ACP5 Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB3948
Key Product Details
Species Reactivity
Validated:
Human
Applications
Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2B Clone # 417603
Product Summary for Human TRACP/PAP/ACP5 Antibody
Immunogen
Mouse myeloma cell line NS0-derived recombinant human TRACP/PAP/ACP5
Ala22-Pro320
Accession # P13686
Ala22-Pro320
Accession # P13686
Specificity
Detects human TRACP in direct ELISAs and Western blots.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2B
Applications for Human TRACP/PAP/ACP5 Antibody
Application
Recommended Usage
Western Blot
1 µg/mL
Sample: Recombinant Human TRACP/PAP/ACP5 (Catalog # 3948-AP)
Sample: Recombinant Human TRACP/PAP/ACP5 (Catalog # 3948-AP)
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: TRACP/PAP/ACP5
Encoded by the ACP5 gene, Tartrate Resistant Acid Phosphatase (TRACP or TRAP) is also known as Purple Acid Phosphatase (PAP) or Acid Phosphatase 5 (ACP5) (1). The deuced amino acid (aa) sequence of human ACP5 predicts a signal peptide (aa 1-21) and a mature chain (aa 22-325). R&D Systems’ recombinant human ACP5 consists of aa 22-320, without the last 5 residues, RRARP.
ACP5 is expressed at high levels by osteoclasts, macrophages and dendritic cells (2). Two forms, 5a and 5b, circulating in human blood, are derived from different cell types and have different functions. Derived from macrophages and dendritic cells, 5a is a marker of inflammatory conditions. Derived from osteoclasts, 5b is a marker of bone resorption. Compared to 5a, 5b does not contain sialic acid residues, has a higher specific activity and pH optimum, and may be processed into a disulfide-linked dimer (3).
References
- Janckila, A.J. and J.M. Halleen (2003) J. Bone Miner. Res. 18:1892.
- Halleen, J.M. et al. (2006) Clin. Lab. 52:499.
- Janckila, A.J. et al. (2003) J. Bone Miner. Res. 18:1916.
Long Name
Tartrate Resistant Acid Phosphatase 5
Alternate Names
ACP5, TRACP-5b, TTRRAP
Gene Symbol
ACP5
UniProt
Additional TRACP/PAP/ACP5 Products
Product Documents for Human TRACP/PAP/ACP5 Antibody
Product Specific Notices for Human TRACP/PAP/ACP5 Antibody
For research use only
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