Jurkat human acute T cell leukemia cell line was stained with Mouse Anti-Human TRAF-6 Monoclonal Antibody (Catalog # MAB3284, filled histogram) or isotype control antibody (Catalog # MAB003, open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.

Applications for Human TRAF-6 Antibody

Application

Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Intracellular Staining by Flow Cytometry

0.25 µg/10⁶ cells
Sample: Jurkat human acute T cell leukemia cell line fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005)

Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: TRAF-6

Tumor Necrosis Factor (TNF) Receptor-Associated Factors (TRAFs) are a family of adaptor proteins that interact with a wide range of cell surface receptors and participate in the regulation of cell survival, proliferation, differentiation, and stress response. TRAFs were identified by their ability to form complexes with TNF receptor superfamily members but more recently are reported to also bind to Toll/IL‑1 receptor family members and mediate cellular signaling. Six members of the TRAF family have been identified. All TRAF proteins have a homologous C-terminal TRAF domain that can bind the cytoplasmic domain of receptors as well as other TRAFs. TRAFs2-6 have N-terminal RING and zinc finger domains that are involved in signaling downstream events. TRAF-6 is a 522 amino acid, 60 kDa protein. Overexpression of TRAF-6 mediates the activation of NF-kappa-B and JNK signaling pathways. TRAF-3 and TRAF-6 have been identified as adaptor molecules involved in TLR/IL-1R signaling events.

Long Name

TNF Receptor-Associated Factor 6

Alternate Names

TRAF6

Entrez Gene IDs

7189 (Human); 22034 (Mouse)

Gene Symbol

TRAF6

UniProt

Q9Y4K3

Additional TRAF-6 Products

Product Documents for Human TRAF-6 Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Human TRAF-6 Antibody

For research use only

Citations
Reviews

Calculators
Flow Cytometry

Human TRAF-6 Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB3284

Key Product Details

Species Reactivity

Applications

Label

Antibody Source

View all TRAF-6 Antibodies »

Product Specifications

Immunogen

Specificity

Clonality

Host

Isotype

Scientific Data Images for Human TRAF-6 Antibody

Detection of TRAF‑6 in Jurkat Human Cell Line by Flow Cytometry.