Human HEK293 cell line transfected with human TRANCE was stained with Mouse Anti-Human TRANCE/TNFSF11/RANK L Monoclonal Antibody (Catalog # MAB6264, filled histogram) or Mouse IgG2B Isotype Control (Catalog # MAB0041, open histogram) followed by anti-Mouse IgG PE-conjugated secondary antibody (Catalog # F0102B). View our protocol for Staining Membrane-associated Proteins.

Applications for Human TRANCE/TNFSF11/RANK L Antibody

Application

Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry

0.25 µg/10⁶ cells
Sample: HEK293 human cell line transfected with human TRANCE and egfp

Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: TRANCE/TNFSF11/RANK L

TNF-related activation-induced cytokine (TRANCE; also RANKL, OPGL, and ODF) is a 35 kDa (predicted) type II transmembrane glycoprotein and member of the TNF cytokine family. Human TRANCE is 317 amino acids (aa) in length and contains a 47 aa cytoplasmic region, a 21 aa transmembrane region, and a 249 extracellular domain (ECD), which contains two potential sites of N-linked glycosylation. Splicing variants produce three isoforms for human TRANCE. Isoform 1 is the standard form. In isoform 2, aa corresponding to 1-73 in isoform 1 are missing. In isoform 3, aa 1-47 are missing. Human TRANCE ECD is 83% identical to mouse TRANCE ECD. TRANCE is expressed highest in the peripheral lymph nodes and weaker in the spleen, peripheral blood leukocytes, bone marrow, heart, placenta, skeletal muscle, stomach, and thyroid. TRANCE plays a role in osteoclast differentiation and activation.

Long Name

TNF-related Activation-induced Cytokine

Alternate Names

CD254, ODF, OPGL, RANK L, RANKL, TNFSF11

Entrez Gene IDs

8600 (Human); 21943 (Mouse); 117516 (Rat); 102137946 (Cynomolgus Monkey)

Gene Symbol

TNFSF11

UniProt

O14788

Additional TRANCE/TNFSF11/RANK L Products

Product Documents for Human TRANCE/TNFSF11/RANK L Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Human TRANCE/TNFSF11/RANK L Antibody

For research use only

Citations
Reviews

Calculators
Flow Cytometry

Human TRANCE/TNFSF11/RANK L Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB6264

Key Product Details

Species Reactivity

Validated:

Applications

Label

Antibody Source

View all TRANCE/TNFSF11/RANK L Antibodies »

Product Summary for Human TRANCE/TNFSF11/RANK L Antibody

Immunogen

Specificity

Clonality

Host

Isotype

Scientific Data Images for Human TRANCE/TNFSF11/RANK L Antibody

Detection of TRANCE/TNFSF11/RANK L in HEK293 human cell line transfected with human TRANCE by Flow Cytometry.