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Human Transgelin/TAGLN Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB78861

R&D Systems, part of Bio-Techne
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MAB78861
MAB78861-SP

Key Product Details

Species Reactivity

Human

Applications

Immunocytochemistry, Simple Western, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 859112

Product Specifications

Immunogen

E. coli-derived recombinant human Transgelin/TAGLN
Ala2-Ser201
Accession # Q01995

Specificity

Detects human Transgelin/TAGLN in ELISAs and Western blots.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Human Transgelin/TAGLN Antibody

Detection of Human and Rat Transgelin/TAGLN antibody by Western Blot.

Detection of Human and Rat Transgelin/TAGLN by Western Blot.

Western blot shows lysates of human aorta tissue, human uterus tissue, and rat uterus tissue. PVDF membrane was probed with 0.5 µg/mL of Mouse Anti-Human Transgelin/TAGLN Monoclonal Antibody (Catalog # MAB78861) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). Specific bands were detected for Transgelin/TAGLN at approximately 16-24 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Transgelin/TAGLN antibody in MCF 10A Human Cell Line by Immunocytochemistry (ICC).

Transgelin/TAGLN in MCF 10A Human Cell Line.

Transgelin/TAGLN was detected in immersion fixed MCF 10A human breast epithelial cell line using Mouse Anti-Human Transgelin/TAGLN Monoclonal Antibody (Catalog # MAB78861) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Detection of Human Transgelin/TAGLN antibody by Simple WesternTM.

Detection of Human Transgelin/TAGLN by Simple WesternTM.

Simple Western lane view shows lysates of human aorta tissue and human uterus tissue, loaded at 0.5 mg/mL. A specific band was detected for Transgelin/TAGLN at approximately 22-25 kDa (as indicated) using 5 µg/mL of Mouse Anti-Human Transgelin/TAGLN Monoclonal Antibody (Catalog # MAB78861). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Applications for Human Transgelin/TAGLN Antibody

Application
Recommended Usage

Immunocytochemistry

8-25 µg/mL
Sample: Immersion fixed MCF 10A human breast epithelial cell line

Simple Western

5 µg/mL
Sample: Human aorta tissue and human uterus tissue

Western Blot

0.5 µg/mL
Sample: Human aorta tissue, human uterus tissue, and rat uterus tissue

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.

Reconstitution Buffer Available:
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Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Transgelin/TAGLN

TAGLN (Transgelin; also 22 kDa Actin-binding protein, WS3-10 and Smooth muscle protein 22 alpha) is a 22-24 kDa cytosolic member of the calponin (calcium-binding and calmodulin-binding troponin T-like protein) family of molecules. It is expressed in both visceral and vascular smooth muscle, fibroblasts, cardiac myocytes, and potentially in breast duct plus prostate epithelium. TAGLN is associated with the actin stress fibers and appears to both suppress MMP-9 production, and downmodulate Ca++-independent smooth muscle contraction. Human TAGLN is 201 amino acids (aa) in length. It contains one CH/calponin homology domain (aa 24-137), and an actin-binding calponin-like repeat/CLIK (aa 175-200). There is one utilized phosphorylation site at Tyr193, plus two utilized acetylation sites in the N-terminus. Three potential isoform variants are reported, one that shows a alternative start site at Met111, a second that contains a new start site 10 aa upstream of the standard site, and a third that possesses a 58 aa substitution for aa 97-201. Full-length human and mouse TAGLN share 97% aa sequence identity.

Alternate Names

SM22 alpha, TAGLN, WS3-10

Entrez Gene IDs

6876 (Human); 21345 (Mouse); 25123 (Rat)

Gene Symbol

TAGLN

UniProt

Additional Transgelin/TAGLN Products

Product Documents for Human Transgelin/TAGLN Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Human Transgelin/TAGLN Antibody

For research use only

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