Human Transglutaminase 4/TGM4 Biotinylated Antibody

R&D Systems, part of Bio-Techne | Catalog # BAF5760

R&D Systems, part of Bio-Techne
Catalog #
Availability
Size / Price
Qty
BAF5760
Print Quote
Bulk Order
Conjugate
Catalog #
Unconjugated
AF5760
View Product
100% Guarantee

Key Product Details

Species Reactivity

Human

Applications

Western Blot

Label

Biotin

Antibody Source

Polyclonal Sheep IgG

View all Transglutaminase 4/TGM4 Antibodies »

Product Specifications

Immunogen

S. frugiperda insect ovarian cell line Sf 21-derived recombinant human Transglutaminase 4/TGM4
Met2-Lys684
Accession # AAC50516

Specificity

Detects human Transglutaminase 4 in Western blots. In Western blots, approximately 5% cross-reactivity with recombinant human (rh) Transglutaminase 2, rhTransglutaminase 3, rhTransglutaminase 7, and recombinant mouse Transglutaminase 2 is observed.

Clonality

Polyclonal

Host

Sheep

Isotype

IgG

Applications for Human Transglutaminase 4/TGM4 Biotinylated Antibody

Application
Recommended Usage

Western Blot

0.1 µg/mL
Sample: Recombinant Human Transglutaminase 4/TGM4

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Reconstitution Buffer Available:
Size / Price
Qty

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Transglutaminase 4/TGM4

TGM4 (Transglutaminase 4; also TGP and Prostate-specific transglutaminase) is a 77 kDa member of the papain-like family of transglutaminases. It is expressed by various tumor cell lines plus prostatic epithelium, and may participate in semen protein modification. TGM4, like other TGMs, can apparently mediate transamidation, plus esterification reactions. Human TGM4 is 684 amino acids (aa) in length. Although it does not contain a signal sequence, based on rodent, it is likely secreted as a homodimer. TGM4 contains an active enzyme site between aa 263‑353, and shows a C-terminal Ig-like domain (aa 587‑684). The molecule has a requirement for divalent calcium. There are two potential splice variants. One shows a 45 aa insertion after Leu8, while another shows a 12 aa substitution for aa 1‑80. Human full‑length TGM4 shares only 54% aa identity with mouse TGM4.

Alternate Names

Fibrinoligase, TGM4, TGP

Entrez Gene IDs

7047 (Human); 331046 (Mouse); 64679 (Rat)

Gene Symbol

TGM4

UniProt

AAC50516

Additional Transglutaminase 4/TGM4 Products

Product Documents for Human Transglutaminase 4/TGM4 Biotinylated Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Human Transglutaminase 4/TGM4 Biotinylated Antibody

For research use only

Privacy and Cookie Policy
Site Map
© Copyright 2024 Bio-Techne. All Rights Reserved.