Human TREM-1 Antibody
R&D Systems, part of Bio-Techne | Catalog # AF1278
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human, Mouse
Applications
Validated:
Agonist Activity, CyTOF-ready, ELISA Capture (Matched Antibody Pair), Flow Cytometry, Western Blot
Cited:
Flow Cytometry, Immunocytochemistry, Immunohistochemistry, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Product Specifications
Immunogen
S. frugiperda insect ovarian cell line Sf 21-derived recombinant human TREM-1
Met1-Arg200
Accession # Q9NP99
Met1-Arg200
Accession # Q9NP99
Specificity
Detects human TREM-1 in ELISAs and Western blots. In ELISAs, less than 0.2% cross-reactivity with recombinant mouse (rm) TREM‑1 and rmTREM‑2b is observed.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Scientific Data Images for Human TREM-1 Antibody
Detection of Human TREM‑1 by Western Blot.
Western blot shows lysates of human granulocytes. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human TREM-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1278) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for TREM-1 at approximately 40 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.Applications for Human TREM-1 Antibody
Application
Recommended Usage
Agonist Activity
Measured by its ability to stimulate TNF-alpha secretion by human peripheral blood mononuclear cells. Bouchon, A. et al. (2001) Nature 410:1103 and Bouchon, A. et al. (2000) J. Immunology 164:4991.
The ED50 for this effect is typically 2 - 6 μg/mL.
The ED50 for this effect is typically 2 - 6 μg/mL.
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
Flow Cytometry
2.5 µg/106 cells
Sample: Human whole blood monocytes and neutrophils
Sample: Human whole blood monocytes and neutrophils
Western Blot
1 µg/mL
Sample: Human granulocytes
Sample: Human granulocytes
Human TREM-1 Sandwich Immunoassay
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: TREM-1
TREM-1 (Triggering Receptor Expressed on Myeloid cells) is a type I transmembrane protein having a single Ig-like domain. It associates with the adapter protein, DAP12, to deliver an activating signal. Several other TREM family members have been reported that are structurally similar but share less than 30% amino acid identity. TREM-1 is expressed on blood neutrophils and a subset of monocytes, and expression is up-regulated by bacterial LPS. The natural ligand for TREM-1 has not been identified. However, engagement of TREM-1 with an agonist monoclonal antibody leads to expression of IL-8, MCP-1, and TNF-alpha, suggesting that this receptor plays an important role in inflammatory responses. TREM-1 is expressed at high levels on neutrophils of patients with microbial sepsis and in mice with
LPS-induced shock. Blockade of TREM-1 with a TREM-1/Fc fusion protein protected mice against LPS-induced shock. Human and mouse TREM-1 share approximately 42% amino acid sequence homology (1-3).
References
- Bouchon, A. (2000) J. Immunol. 164:4991.
- Bouchon, A. (2001) Nature 410:1103.
- Nathan, C. and A. Ding (2001) Nature Med. 7:530.
Long Name
Triggering Receptor Expressed on Myeloid Cells 1
Alternate Names
CD354, TREM1
Gene Symbol
TREM1
UniProt
Additional TREM-1 Products
Product Documents for Human TREM-1 Antibody
Product Specific Notices for Human TREM-1 Antibody
For research use only
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