Human TRF2 Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB5635

R&D Systems, part of Bio-Techne
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MAB5635
MAB5635-SP
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Key Product Details

Species Reactivity

Human

Applications

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 689524

View all TRF-2 Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human TRF2
Gln78-Thr238 (predicted)
Accession # Q15554

Specificity

Detects human TRF2 in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human TRF1 is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Human TRF2 Antibody

Detection of Human TRF2 antibody by Western Blot.

Detection of Human TRF2 by Western Blot.

Western blot shows lysates of Daudi human Burkitt's lymphoma cell line, Jurkat human acute T cell leukemia cell line, and Raji human Burkitt's lymphoma cell line. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human TRF2 Monoclonal Antibody (Catalog # MAB5635) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for TRF2 at approximately 75 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Applications for Human TRF2 Antibody

Application
Recommended Usage

Western Blot

1 µg/mL
Sample: Daudi human Burkitt's lymphoma cell line, Jurkat human acute T cell leukemia cell line, and Raji human Burkitt's lymphoma cell line
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.

Reconstitution Buffer Available:
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Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: TRF-2

TRF2 (telomeric repeat-binding factor 2; also telomeric DNA-binding protein) is a 55-60 kDa, ubiquitously expressed nuclear protein that participates in telomere homeostasis. TRF-2 binds as a dimer to TTAGGG repeats at ends of chromosomes (telomeres), where it blocks inappropriate activation of the ATM/p53 pathway. It also collaborates with TRF1 to promote normal telomere length. Human TRF2 is 500 amino acids (aa) in length. It contains an N-terminal Arg-rich region (aa 13-30), a dimerization domain (aa 46-112), an NLS (aa 329-333), and a DNA binding HTH myb-type domain (aa 442-499). There is one potential alternate start site 42 aa upstream of the standard start site, and one splice form that shows a 13 aa substitution for aa 239-500. Over aa 78-238, human TRF2 shares 96% aa identity with mouse TRF2.

Long Name

Telomeric Repeat Binding Factor 2

Alternate Names

TERF2, TRBF2, TRF2

Entrez Gene IDs

7014 (Human); 21750 (Mouse); 361403 (Rat)

Gene Symbol

TERF2

UniProt

Q15554

Additional TRF-2 Products

Product Documents for Human TRF2 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human TRF2 Antibody

For research use only

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