Human TROP-2 Biotinylated Antibody
R&D Systems, part of Bio-Techne | Catalog # BAF650
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human
Applications
Validated:
Immunohistochemistry, Western Blot
Cited:
ELISA Detection, Flow Cytometry, Functional Assay, Immunohistochemistry, Western Blot
Label
Biotin
Antibody Source
Polyclonal Goat IgG
Product Specifications
Immunogen
Mouse myeloma cell line NS0-derived recombinant human TROP-2
Thr88-Thr274
Accession # P09758
Thr88-Thr274
Accession # P09758
Specificity
Detects human TROP-2 in Western blots. In Western blots, less than 1% cross-reactivity with recombinant human E‑Cadherin is observed.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for Human TROP-2 Biotinylated Antibody
TROP-2 in Human Cervical Cancer Tissue.
TROP-2 was detected in immersion fixed paraffin-embedded sections of human cervical cancer tissue using Goat Anti-Human TROP-2 Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF650) at 3 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Goat IgG VisUCyte™ HRP Polymer Antibody (brown; Catalog # VC004) and counterstained with hematoxylin (blue). Specific staining was localized to cell membrane in cancer cells. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.Applications for Human TROP-2 Biotinylated Antibody
Application
Recommended Usage
Immunohistochemistry
3-15 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human cervical cancer tissue
Sample: Immersion fixed paraffin-embedded sections of human cervical cancer tissue
Western Blot
0.1 µg/mL
Sample: Recombinant Human TROP-2 Fc Chimera (Catalog # 650-T2)
Sample: Recombinant Human TROP-2 Fc Chimera (Catalog # 650-T2)
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: TROP-2
Human TROP-2, also called tumor associated calcium signal transducer 2 (TACSTD2), GA733-1, gp50 and T16, is a type I cell surface glycoprotein that is highly expressed on human carcinomas. It was originally identified as an antigen present on human gastrointestinal tumors and is the second of two members of this family. The other family member is GA733-2, also called EpCAM, TROP-1, 17-1A, gp40 and KSA. The TROP-2 gene is unique in that it contains no introns. A study of these two genes suggested that TROP-2 was the result of a retroposition of the EpCAM gene. TROP-2 and EpCAM share approximately 49% amino acid identity and approximately 67% similarity. Human and mouse TROP-2 share 87% similarity. The human TROP-2 protein consists of a putative 26 amino acid (aa) signal sequence, a 248 aa extracellular domain, a 23 aa transmembrane region and a 26 aa cytoplasmic domain. TROP-2 is capable of transducing an intracellular calcium signal and may play a role in tumor growth. It also has adhesive functions.
References
- Linnenbach, A.J. et al. (1989) Proc. Natl. Acad. Sci. USA 86:27.
- Linnenbach, A.J. et al. (1993) Mol. Cell. Biol. 13:1507.
- Fornaro, M. et al. (1995) Int. J. Cancer 62:610.
- Ripani, E. et al. (1998) Int. J. Cancer 76:671.
- El Sewedy, T. et al. (1998) Int. J. Cancer 75:324.
Long Name
Tumor-associated Calcium Signal Transducer 2
Alternate Names
GA733-1, gp50, T16, TACSTD2, TROP2
Gene Symbol
TACSTD2
UniProt
Additional TROP-2 Products
Product Documents for Human TROP-2 Biotinylated Antibody
Product Specific Notices for Human TROP-2 Biotinylated Antibody
For research use only
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