Human TROP-2 Fluorescein-conjugated Antibody
R&D Systems, part of Bio-Techne | Catalog # FAB650F
Conjugate
Catalog #
Key Product Details
Species Reactivity
Human
Applications
Flow Cytometry
Label
Fluorescein (Excitation = 488 nm, Emission = 515-545 nm)
Antibody Source
Monoclonal Mouse IgG2A Clone # 77220
Product Specifications
Immunogen
Mouse myeloma cell line NS0-derived recombinant human TROP-2
His27-Thr274
Accession # P09758
His27-Thr274
Accession # P09758
Specificity
Detects human TROP-2 in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant human (rh) VCAM‑1 or rhICAM‑1 is observed.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2A
Scientific Data Images for Human TROP-2 Fluorescein-conjugated Antibody
Detection of TROP‑2 in PC‑3 Human Cell Line by Flow Cytometry.
PC-3 human prostate cancer cell line was stained with Mouse Anti-Human TROP-2 Fluorescein-conjugated Monoclonal Antibody (Catalog # FAB650F, filled histogram) or isotype control antibody (Catalog # IC003F, open histogram). View our protocol for Staining Membrane-associated Proteins.Applications for Human TROP-2 Fluorescein-conjugated Antibody
Application
Recommended Usage
Flow Cytometry
10 µL/106 cells
Sample: PC‑3 human prostate cancer cell line
Sample: PC‑3 human prostate cancer cell line
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8° C. Do not use past expiration date. Protect from light.
Background: TROP-2
Human TROP-2, also known as Tumor Associated Calcium Signal Transducer 2 (TACSTD2), GA733-1, gp50 and T16, is a type I cell surface glycoprotein that is highly expressed on human carcinomas. It was originally identified as an antigen present on human gastrointestinal tumors and is the second of two members of this family. The other family member is GA733-2, also called EpCAM, TROP-1, 17-1A, gp40 and KSA. The TROP-2 gene is unique in that it contains no introns. A study of these two genes suggested that TROP-2 was the result of a retroposition of the EpCAM gene. TROP-2 and EpCAM share approximately 49% amino acid sequence identity and approximately 67% similarity. Human and mouse TROP-2 share 87% similarity. The human TROP-2 protein consists of a putative 26 amino acid (aa) signal sequence, a 248 aa extracellular domain, a 23 aa transmembrane region and a 26 aa cytoplasmic domain. TROP-2 is capable of transducing an intracellular calcium signal and may play a role in tumor growth. It also has adhesive functions.
References
- Linnenbach, A.J. et al. (1989) Proc. Natl. Acad. Sci. USA 86:27.
- Linnenbach, A.J. et al. (1993) Mol. Cell. Biol. 13:1507.
- Fornaro, M. et al. (1995) Int. J. Cancer 62:610.
- Ripani, E. et al. (1998) Int. J. Cancer 76:671.
- El Sewedy, T. et al. (1998) Int. J. Cancer 75:324.
Long Name
Tumor-associated Calcium Signal Transducer 2
Alternate Names
GA733-1, gp50, T16, TACSTD2, TROP2
Gene Symbol
TACSTD2
UniProt
Additional TROP-2 Products
Product Documents for Human TROP-2 Fluorescein-conjugated Antibody
Product Specific Notices for Human TROP-2 Fluorescein-conjugated Antibody
For research use only
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