Detects human TROP-2 in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant human (rh) VCAM‑1 or rhICAM‑1 is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG_2A

Scientific Data Images for Human TROP-2 PE-conjugated Antibody

Detection of TROP-2 in PC-3 Human Cell Line by Flow Cytometry.

PC-3 human prostate cancer cell line was stained with Mouse Anti-Human TROP-2 PE-conjugated Monoclonal Antibody (Catalog # FAB650P, filled histogram) or isotype control antibody (Catalog # IC003P, open histogram). View our protocol for Staining Membrane-associated Proteins.

Applications for Human TROP-2 PE-conjugated Antibody

Application

Recommended Usage

Flow Cytometry

10 µL/10⁶ cells
Sample: PC‑3 human prostate cancer cell line

Reviewed Applications

Read 1 review rated 1 using FAB650P in the following applications:

Flow Cytometry (1 Review)

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.

12 months from date of receipt, 2 to 8 °C as supplied.

Background: TROP-2

Human TROP-2, also known as Tumor Associated Calcium Signal Transducer 2 (TACSTD2), GA733-1, gp50 and T16, is a type I cell surface glycoprotein that is highly expressed on human carcinomas. It was originally identified as an antigen present on human gastrointestinal tumors and is the second of two members of this family. The other family member is GA733-2, also called EpCAM, TROP-1, 17-1A, gp40 and KSA. The TROP-2 gene is unique in that it contains no introns. A study of these two genes suggested that TROP-2 was the result of a retroposition of the EpCAM gene. TROP-2 and EpCAM share approximately 49% amino acid identity and approximately 67% similarity. Human and mouse TROP-2 share 87% similarity. The human TROP-2 protein consists of a putative 26 amino acid (aa) signal sequence, a 248 aa extracellular domain, a 23 aa transmembrane region and a 26 aa cytoplasmic domain. TROP-2 is capable of transducing an intracellular calcium signal and may play a role in tumor growth. It also has adhesive functions.

References

Linnenbach, A.J. et al. (1989) Proc. Natl. Acad. Sci. USA 86:27.
Linnenbach, A.J. et al. (1993) Mol. Cell. Biol. 13:1507.
Fornaro, M. et al. (1995) Int. J. Cancer 62:610.
Ripani, E. et al. (1998) Int. J. Cancer 76:671.
El Sewedy, T. et al. (1998) Int. J. Cancer 75:324.

Long Name

Tumor-associated Calcium Signal Transducer 2

Alternate Names

GA733-1, gp50, T16, TACSTD2, TROP2

Entrez Gene IDs

4070 (Human); 56753 (Mouse)

Gene Symbol

TACSTD2

UniProt

P09758

Additional TROP-2 Products

Product Documents for Human TROP-2 PE-conjugated Antibody

Product Datasheets

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COA

SDS

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Product Specific Notices for Human TROP-2 PE-conjugated Antibody

For research use only

Citations
Reviews

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Flow Cytometry

Human TROP-2 PE-conjugated Antibody

R&D Systems, part of Bio-Techne | Catalog # FAB650P

Key Product Details

Species Reactivity

Validated:

Cited:

Applications