Human TSHR Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB6534
Key Product Details
Species Reactivity
Human
Applications
ELISA, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2A Clone # 484405
Product Specifications
Immunogen
NS0 mouse myeloma cell line transfected with human TSH R
Accession # P16473
Accession # P16473
Specificity
Detects human TSH R in direct ELISAs and Western blots.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2A
Scientific Data Images for Human TSHR Antibody
Detection of Human TSH R by Western Blot.
Western blot shows lysates of human thyroid tissue. PVDF Membrane was probed with 2 µg/mL of Mouse Anti-Human TSH R Monoclonal Antibody (Catalog # MAB6534) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). Specific bands were detected for TSH R at approximately 55 and 83 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 7.
Human TSH R ELISA Standard Curve.
Recombinant human TSH R protein was serially diluted 2-fold and captured by Mouse Anti-Human TSH R Monoclonal Antibody (Catalog # MAB65341) coated on a Clear Polystyrene Microplate (Catalog # DY990). Mouse Anti-Human TSH R Monoclonal Antibody (Catalog # MAB6534) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998) followed by Substrate Solution (Catalog # DY999) and stopping the enzymatic reaction with Stop Solution (Catalog # DY994).Applications for Human TSHR Antibody
Application
Recommended Usage
ELISA
This antibody functions as an ELISA detection antibody when paired with Mouse Anti-Human TSH R Monoclonal Antibody (Catalog # MAB65341).
This product is intended for assay development on various assay platforms requiring antibody pairs.
Western Blot
2 µg/mL
Sample: Human thyroid tissue
Sample: Human thyroid tissue
Formulation, Preparation, and Storage
Purification
Protein A or G purified from ascites
Reconstitution
Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: TSHR
Thyroid stimulating hormone receptor (TSH R; also thyrotropin receptor) is an 85 kDa (unglycosylated) member of the G-protein coupled receptor 1 family. Human TSH R is synthesized as a 764 amino acid (aa) precursor that contains a 20 aa signal sequence, a 393 aa extracellular domain (ECD), a 269 aa membrane spanning domain consisting of seven transmembrane segments, and an 82 aa cytoplasmic domain. The ECD contains six leucine-rich repeats (LRR) and six potential sites of N-linked glycosylation. There are two isoforms, long and short, produced by a splicing variant corresponding to aa 253-764 in the long isoform that is missing in the short isoform. Human TSH R is 86-87% aa identical to mouse and rat TSH R. TSH R is the receptor for thyroid stimulating hormone, which plays a central role in controlling thyroid cell metabolism.
Long Name
Thyroid Stimulating Hormone Receptor
Alternate Names
CHNG1, LGR3, TSH R
Gene Symbol
TSHR
UniProt
Additional TSHR Products
Product Documents for Human TSHR Antibody
Product Specific Notices for Human TSHR Antibody
For research use only
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