HT-29 human colon adenocarcinoma cell line was stained with Mouse Anti-Human TSPAN1 Monoclonal Antibody (Catalog # MAB9186, filled histogram) or isotype control antibody (Catalog # MAB007, open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). View our protocol for Staining Membrane-associated Proteins.

Detection of TSPAN1 in HEK293 Human Cell Line Transfected with Human TSPAN1 and eGFP by Flow Cytometry.

HEK293 human embryonic kidney cell line transfected with either (A) human TSPAN1 or (B) irrelevant transfectants and eGFP was stained with Mouse Anti-Human TSPAN1 Monoclonal Antibody (Catalog # MAB9186) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). Quadrant markers were set based on control antibody staining (Catalog # MAB007). View our protocol for Staining Membrane-associated Proteins.

Applications for Human TSPAN1 Antibody

Application

Recommended Usage

Flow Cytometry

0.25 µg/10⁶ cells
Sample: HT‑29 human colon adenocarcinoma cell line and HEK293 human embryonic kidney cell line transfected with human TSPAN1

Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in TBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: TSPAN1

Tetraspanins (TSPAN family) are small integral proteins that contain four transmembrane domains with two different and well characterized extracellular domains. Tetraspanins are found on the plasma membrane and within various intracellular organelles and granules in nearly all cell and tissue types. They interact with one another and with other transmembrane molecules, including integrins, generating a ‘web' of interactions believed to control cell and membrane trafficking and compartmentalization. Tetraspanin-1 (TSPAN1), a 241 amino acid protein, interacts with the thiamine transporter-1 (THTR-1, SLC19A2) in human intestinal epithelial cells, linking TSPAN1 to the regulation of thiamine uptake. TSPAN1 has been found to be expressed in gastric, ovarian, colon, and other carcinomas.

Long Name

Tetraspanin 1

Alternate Names

NET1, TM4C, TM4SF

Entrez Gene IDs

10103 (Human); 66805 (Mouse); 298436 (Rat)

Gene Symbol

TSPAN1

UniProt

O60635 (Human)

Additional TSPAN1 Products

Product Documents for Human TSPAN1 Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Human TSPAN1 Antibody

For research use only

Citations
Reviews

Calculators
Flow Cytometry

Human TSPAN1 Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB9186

Key Product Details

Species Reactivity

Validated:

Applications

Label

Antibody Source

View all TSPAN1 Antibodies »

Product Summary for Human TSPAN1 Antibody

Immunogen

Specificity

Clonality

Host

Isotype

Scientific Data Images for Human TSPAN1 Antibody

Detection of TSPAN1 in HT‑29 Human Cell Line by Flow Cytometry.