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Human TSPAN12 Alexa Fluor® 647-conjugated Antibody

R&D Systems, part of Bio-Techne | Catalog # FAB8910R

R&D Systems, part of Bio-Techne

Key Product Details

Species Reactivity

Human

Applications

Flow Cytometry

Label

Alexa Fluor 647 (Excitation = 650 nm, Emission = 668 nm)

Antibody Source

Monoclonal Mouse IgG2B Clone # 921938

Product Specifications

Immunogen

NS0 mouse myeloma cell line transfected with human TSPAN12
Met1-Leu305
Accession # O95859

Specificity

Stains human TSPAN12 transfectants but not irrelevant transfectants in flow cytometry. 

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Scientific Data Images for Human TSPAN12 Alexa Fluor® 647-conjugated Antibody

Detection of TSPAN12 antibody in HEK293 Human Cell Line Transfected with Human TSPAN12 and eGFP antibody by Flow Cytometry.

Detection of TSPAN12 in HEK293 Human Cell Line Transfected with Human TSPAN12 and eGFP by Flow Cytometry.

HEK293 human embryonic kidney cell line transfected with (A) human TSPAN12 or (B) irrelevant transfectants and eGFP was stained with Mouse Anti-Human TSPAN12 Alexa Fluor® 647-conjugated Monoclonal Antibody (Catalog # FAB8910R). Quadrant markers were set based on control antibody staining (Catalog # IC0041R). View our protocol for Staining Membrane-associated Proteins.

Applications for Human TSPAN12 Alexa Fluor® 647-conjugated Antibody

Application
Recommended Usage

Flow Cytometry

5 µL/106 cells
Sample: HEK293 human embryonic kidney cell line transfected with human TSPAN12 and eGFP

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: TSPAN12

TSPAN12 is a member of the transmembrane 4 superfamily, also known as the tetraspanin family. They mediate signal transduction events that play a role in the regulation of cell development, activation, growth and motility. TSPAN12 plays a central role in retinal vascularization by regulating norrin signal transduction. TSPAN12 acts in concert with norrin to promote FZD4 multimerization and subsequent activation of FZD4, leading to promote accumulation of beta-catenin and stimulate LEF/TCF-mediated transcriptional programs. Defects in TSPAN12 are the cause of vitreoretinopathy exudative type 5, a disorder of the retinal vasculature characterized by an abrupt cessation of growth of peripheral capillaries, leading to an avascular peripheral retina.

Long Name

Tetraspanin 12

Alternate Names

EVR5, NET2, TM4SF12

Entrez Gene IDs

23554 (Human); 269831 (Mouse); 362326 (Rat)

Gene Symbol

TSPAN12

UniProt

Additional TSPAN12 Products

Product Documents

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Human TSPAN12 Alexa Fluor® 647-conjugated Antibody


This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.

For research use only

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