HEK293 human embryonic kidney cell line either transfected with (A) human TSPAN7 or (B) irrelevant transfectants and eGFP was stained with Mouse Anti-Human TSPAN7 Monoclonal Antibody (Catalog # MAB9179) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). Quadrant markers were set based on control antibody staining (Catalog # MAB003). View our protocol for Staining Membrane-associated Proteins.

Applications for Human TSPAN7 Antibody

Application

Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry

0.25 µg/10⁶ cells
Sample: HEK293 human embryonic kidney cell line transfected with human TSPAN7 and eGFP

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: TSPAN7

Tetraspanin-7 (aka TSPAN7, TALLA-1, TM4SF2 and CD231) is a 249 aminoacids (aa) multi-pass membrane protein. Tetraspanin proteins regulate morphology, signaling and trafficking processes by interaction and association with proteins in tetraspanin enriched microdomains (TEMs). It has been proposed that tetraspanins interact with integrins to affect cell migration, probably by modulation or compartmentalization of integrin signaling. TSPAN7 has been found to be upregulated in myeloma development and described as a prognostic marker of renal cell carcinoma. Mutations in TSPAN7 are also implicated in some forms of X-linked intellectual disability.

Long Name

Tetraspanin 7

Alternate Names

A15, CCG-B7, CD231, DXS1692E, MRX58, MXS1, TALLA-1, TM4SF2, TM4SF2b

Entrez Gene IDs

7102 (Human); 21912 (Mouse); 363447 (Rat)

Gene Symbol

TSPAN7

UniProt

P41732 (Human)

Additional TSPAN7 Products

Product Documents for Human TSPAN7 Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Human TSPAN7 Antibody

For research use only

Citations
Reviews

Calculators
Flow Cytometry

Human TSPAN7 Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB9179

Key Product Details

Species Reactivity

Applications

Label

Antibody Source

View all TSPAN7 Antibodies »

Product Specifications

Immunogen

Specificity

Clonality

Host

Isotype

Scientific Data Images for Human TSPAN7 Antibody

Detection of TSPAN7 in HEK293 Human Cell Line Transfected with Human TSPAN7 and eGFP by Flow Cytometry.