Western blot shows lysates of COLO 205 human colorectal adenocarcinoma cell line and HT-29 human colon adenocarcinoma cell line. PVDF membrane was probed with 2 µg/mL of Rat Anti-Human TSPAN8 Monoclonal Antibody (Catalog # MAB4734) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (HAF005). A specific band was detected for TSPAN8 at approximately 35 kDa (as indicated). This experiment was conducted under non-reducing conditions and using Immunoblot Buffer Group 1.

Detection of TSPAN8 in HT-29 Human Cell Line by Flow Cytometry.

HT-29 human colon adenocarcinoma cell line was stained with Rat Anti-Human TSPAN8 Monoclonal Antibody (Catalog # MAB4734, filled histogram) or isotype control antibody (MAB0061, open histogram), followed by Allophycocyanin-conjugated Anti-Rat IgG F(ab')₂Secondary Antibody (Catalog # F0113). View our protocol for Staining Membrane-associated Proteins.

TSPAN8 in HT‑29 Human Cell Line.

TSPAN8 was detected in immersion fixed HT-29 human colon adenocarcinoma cell line using Rat Anti-Human TSPAN8 Monoclonal Antibody (Catalog # MAB4734) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rat IgG Secondary Antibody (red; NL013) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

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Applications for Human TSPAN8 Antibody

Application

Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry

0.25 µg/10⁶ cells
Sample: HT-29 human colon adenocarcinoma cell line

Immunocytochemistry

3-25 µg/mL
Sample: Immersion fixed HT‑29 Human Colon Adenocarcinoma Cell Line (Positive) and Daudi Human Burkitt's Lymphoma Cell Line (Negative) Cells

Western Blot

2 µg/mL
Sample: COLO 205 human colorectal adenocarcinoma cell line and HT‑29 human colon adenocarcinoma cell line

Reviewed Applications

Read 2 reviews rated 4.5 using MAB4734 in the following applications:

Immunocytochemistry/Immunofluorescence (1 Review)
Western Blot (1 Review)

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: TSPAN8

Tetraspanin-8 (TSPAN8), also known as TM4SF3 and human tumor-associated antigen CO-0029, is a member of the transmembrane 4 superfamily. It is a cell surface 27‑34 kDa glycoprotein with 4 transmembrane segments, and a large extracellular loop (LEL) domain from amino acids (aa) 106‑206. Both the N- and C-termini are intracellular. TSPAN8 is expressed by multiple tumor types as well as smooth and skeletal muscle cells, endothelial cells, hematopoietic progenitor cells and non-keratinized squamous epithelium. As with other TSPAN family molecules, TSPAN8 acts as an organizer of microdomains in membranes. Molecules that may be found in these domains include CD151 (another TSPAN family member), beta1 Integrins, Integrin alpha4 beta6, EpCAM, and CD13. TSPAN8 would appear to remove Integrins from the cell surface, facilitating cell motility which, in the case of tumor cells, results in metastasis. TSPAN8 shares 53% aa sequence identity with both mouse and rat TSPAN8.

Long Name

Tetraspanin 8/Transmembrane 4 Superfamily, Member 3

Alternate Names

CO-029, TM4SF3

Entrez Gene IDs

7103 (Human); 216350 (Mouse); 171048 (Rat)

Gene Symbol

TSPAN8

UniProt

AAH05246

Additional TSPAN8 Products

Product Documents for Human TSPAN8 Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Human TSPAN8 Antibody

For research use only

Citations
Reviews

Calculators
Flow Cytometry

Human TSPAN8 Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB4734

Key Product Details

Species Reactivity

Validated:

Cited:

Applications

Validated:

Cited:

Label

Antibody Source

View all TSPAN8 Antibodies »

Product Specifications

Immunogen

Specificity

Clonality

Host

Isotype

Scientific Data Images for Human TSPAN8 Antibody

Detection of Human TSPAN8 by Western Blot.