Human Twist-1 Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB6230R
Recombinant Monoclonal Antibody.
Conjugate
Catalog #
Key Product Details
Species Reactivity
Validated:
Human
Applications
CyTOF-ready, Intracellular Staining by Flow Cytometry, Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Mouse IgG1 Clone # 927403R
Product Summary for Human Twist-1 Antibody
Immunogen
E.coli-derived recombinant human Twist-1
Met1-His202
Accession # Q15672
Met1-His202
Accession # Q15672
Specificity
Detects human Twist-1 in direct ELISAs.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Scientific Data Images for Human Twist-1 Antibody
Detection of Human Twist‑1 by Western Blot.
Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line and PC-3 human prostate cancer cell line. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human Twist-1 Monoclonal Antibody (Catalog # MAB6230R) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for Twist-1 at approximately 23 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Detection of Twist‑1 in A549 Human Cell Line by Flow Cytometry.
A549 human lung carcinoma cell line was stained with Mouse Anti-Human Twist-1 Monoclonal Antibody (Catalog # MAB6230R, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram) followed by PE-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B). To facilitate intracellular staining, cells were fixed and permeabilized with FlowX FoxP3 Fixation & Permeabilization Buffer Kit (Catalog # FC012). View our protocol for Staining Intracellular Molecules.Applications for Human Twist-1 Antibody
Application
Recommended Usage
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
Intracellular Staining by Flow Cytometry
0.25 µg/106 cells
Sample: A549 human lung carcinoma cell line fixed and permeabilized with FlowX FoxP3 Fixation & Permeabilization Buffer Kit (Catalog # FC012)
Sample: A549 human lung carcinoma cell line fixed and permeabilized with FlowX FoxP3 Fixation & Permeabilization Buffer Kit (Catalog # FC012)
Western Blot
2 µg/mL
Sample: HeLa human cervical epithelial carcinoma cell line and PC‑3 human prostate cancer cell line
Sample: HeLa human cervical epithelial carcinoma cell line and PC‑3 human prostate cancer cell line
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Reviewed Applications
Read 1 review rated 5 using MAB6230R in the following applications:
Formulation, Preparation, and Storage
Purification
Protein A or G purified from cell culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Twist-1
Twist-1 (Twist-related protein 1; also H-Twist and bHLHa38) is a 25‑27 kDa class B member of the bHLH transcription factor family of proteins. It is widely expressed in embryo, and select adult cells such as white adipocytes. In fat, Twist-1 induces fatty acid oxidation via PGC-1 alpha and CPT-1, and promotes MCP-1 and TNF-alpha secretion by adipocytes. In epithelium, Twist-1 dysregulation represses E-Cadherin and induces N-Cadherin expression, resulting in an epithelial-to-mesenchymal transition that can lead to cancer. Human Twist-1 is 202 amino acids (aa) in length. It contains a bHLH domain (aa 109-164) with an embedded DNA-binding motif. Twist-1 forms homodimers, and heterodimerizes with TCF3, HAND 1 and HAND 2. Two distinct mutations exist that may impact its dimerization pattern. There is one seven aa insertion after Ile135, and a second seven aa insertion after Pro139. A third unrelated variant shows a four Gly insert after Gly92. Full-length human Twist-1 shares 98% aa identity with mouse Twist-1.
Long Name
Twist Related Protein 1 [Drosophila]
Alternate Names
ACS3, BPES3, H-twist, Twist1
Gene Symbol
TWIST1
UniProt
Additional Twist-1 Products
Product Documents for Human Twist-1 Antibody
Product Specific Notices for Human Twist-1 Antibody
For research use only
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