Detects human Dtk in ELISAs and Western blots. In ELISAs and Western blots, no cross-reactivity or interference with recombinant mouse Dtk, recombinant human (rh) Axl, or rhMer is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG₁

Scientific Data Images for Human Tyro3/Dtk PE-conjugated Antibody

Detection of Dtk in K562 Human Cell Line by Flow Cytometry.

K562 human chronic myelogenous leukemia cell line was stained with Mouse Anti-Human Dtk PE-conjugated Monoclonal Antibody (Catalog # FAB859P, filled histogram) or isotype control antibody (Catalog # IC002P, open histogram). View our protocol for Staining Membrane-associated Proteins.

Applications for Human Tyro3/Dtk PE-conjugated Antibody

Application

Recommended Usage

Flow Cytometry

10 µL/10⁶ cells
Sample: K562 human chronic myelogenous leukemia cell line

Reviewed Applications

Read 2 reviews rated 5 using FAB859P in the following applications:

Flow Cytometry (2 Reviews)

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.

12 months from date of receipt, 2 to 8 °C as supplied.

Background: Tyro3/Dtk

Dtk (also known as Sky, Tyro3, Rse, Brt), Axl (also known as Ufo, Ark) and Mer (human and mouse homologues of chicken c-Eyk) constitute a receptor tyrosine kinase subfamily. The extracellular domain of these proteins contain two Ig-like motifs and two fibronectin type III motifs. This characteristic topology is also found in neural cell adhesion molecules and in receptor tyrosine phosphatases. All three receptors bind the vitamin K-dependent protein growth-arrest specific gene 6 (Gas6) which is structurally related to the anticoagulation factor Protein S. The binding affinities for Gas6 are in the order of Axl > Dtk > Mer. Gas6 binding induces tyrosine phosphorylation and downstream signaling pathways that can lead to cell proliferation, migration, or the prevention of apoptosis. Dtk is widely expressed during embryonic development. In adults, Dtk is predominantly expressed in neurons in restricted regions of the brain.

References

Nagata, K. et al. (1996) J. Biol. Chem. 22:30022.
Crosier, K.E. and P.S Crosier (1997) Pathology 29:131.

Long Name

Tyrosine Protein Kinase Receptor TYRO3

Alternate Names

Brt, Dtk, Rse, Sky

Entrez Gene IDs

7301 (Human); 22174 (Mouse); 102135978 (Cynomolgus Monkey)

Gene Symbol

TYRO3

UniProt

Q06418

Additional Tyro3/Dtk Products

Product Documents for Human Tyro3/Dtk PE-conjugated Antibody

Product Datasheets

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COA

SDS

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Product Specific Notices for Human Tyro3/Dtk PE-conjugated Antibody

For research use only

Citations
Reviews

Spectra Viewer
Flow Cytometry

Human Tyro3/Dtk PE-conjugated Antibody

R&D Systems, part of Bio-Techne | Catalog # FAB859P

Key Product Details

Species Reactivity

Validated:

Cited:

Applications