Human UCH-L1/PGP9.5 Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB6007

R&D Systems, part of Bio-Techne
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MAB6007
MAB6007-SP
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Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Immunohistochemistry, Immunoprecipitation, Knockout Validated, Simple Western, Western Blot

Cited:

Immunohistochemistry, Immunoprecipitation, Surface Plasmon Resonance (SPR, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # 671108

View all UCH-L1/PGP9.5 Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human UCH-L1/PGP9.5
Gln2-Ala223
Accession # P09936

Specificity

Detects human UCH-L1/PGP9.5 in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant human UCH-L3 is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Scientific Data Images for Human UCH-L1/PGP9.5 Antibody

Detection of Human UCH-L1/PGP9.5 antibody by Western Blot.

Detection of Human UCH-L1/PGP9.5 by Western Blot.

Western blot shows lysates of human brain (cortex) tissue. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human UCH-L1/PGP9.5 Monoclonal Antibody (Catalog # MAB6007) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for UCH-L1/PGP9.5 at approximately 29 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
UCH-L1/PGP9 by Immunohistochemistry (IHC-P).5 antibody in Human Prostate by Immunohistochemistry (IHC-P).

UCH-L1/PGP9.5 in Human Prostate.

UCH-L1/PGP9.5 was detected in immersion fixed paraffin-embedded sections of human prostate using Mouse Anti-Human UCH-L1/PGP9.5 Monoclonal Antibody (Catalog # MAB6007) at 15 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm of glandular epithelial cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Detection of Human and Mouse UCH-L1/PGP9.5 antibody by Simple WesternTM.

Detection of Human and Mouse UCH-L1/PGP9.5 by Simple WesternTM.

Simple Western lane view shows lysates of human brain (cortex) tissue and mouse brain (cortex) tissue, loaded at 0.2 mg/mL. A specific band was detected for UCH-L1/PGP9.5 at approximately 32 kDa (as indicated) using 10 µg/mL of Mouse Anti-Human UCH-L1/PGP9.5 Monoclonal Antibody (Catalog # MAB6007). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
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Applications for Human UCH-L1/PGP9.5 Antibody

Application
Recommended Usage

Immunohistochemistry

8-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human prostate

Immunoprecipitation

25 µg/mL
Sample: Cell lysates spiked with Recombinant Human UCH-L1/PGP9.5 (Catalog # 6007-CY), see our available Western blot detection antibodies

Knockout Validated

UCH-L1/PGP9.5 is specifically detected in HEK293T human embryonic kidney parental cell line but is not detectable in UCH-L1/PGP9.5 knockout HEK293T cell line.

Simple Western

10 µg/mL
Sample: Human brain (cortex) tissue and mouse brain (cortex) tissue

Western Blot

2 µg/mL
Sample: Human brain (cortex) tissue

Reviewed Applications

Read 2 reviews rated 4.5 using MAB6007 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.

Reconstitution Buffer Available:
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Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: UCH-L1/PGP9.5

UCH-L1 (ubiquitin carboxyterminal hydrolase isozyme 1; also PGP9.5) is a 24-27 kDa member of the peptidase C12 family of enzymes. It shows restricted expression, being found in neurons and oocytes. UCH-L1 has dual enzymatic activity. As a monomer, it is a ubiquitin hydrolase that removes ubiquitin from modified proteins; as a homodimer, it acts as a ligase that creates ubiquitin dimers. In neurons, UCH-L1’s most important role appears to be that of generating free ubiquitin. Human UCH-L1 is 223 amino acids (aa) in length. It is O-glycosylated, ubiquitinated, and farnesylated; when farnesylated, it becomes associated with cell membranes. Three potential splice forms are reported. One shows a two aa substitution for aa 12-15, a second contains an alternative start site at Met82, and a third shows the same start site coupled with a deletion of aa 138-153. Full-length human UCH-L1 shares 95% aa identity with mouse UCH-L1.       

Long Name

Ubiquitin C-terminal Hydrolase L1

Alternate Names

PARK5, PGP9.5, UCHL1

Entrez Gene IDs

7345 (Human); 22223 (Mouse)

Gene Symbol

UCHL1

UniProt

P09936 (Human)

Additional UCH-L1/PGP9.5 Products

Product Documents for Human UCH-L1/PGP9.5 Antibody

Product Datasheets

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human UCH-L1/PGP9.5 Antibody

For research use only

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