Human UCH-L3 Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB6008
Key Product Details
Species Reactivity
Human
Applications
Immunoprecipitation, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # 677710
Product Specifications
Immunogen
E. coli-derived recombinant human UCH-L3
Glu2-Ala230
Accession # P15374
Glu2-Ala230
Accession # P15374
Specificity
Detects human UCH-L3 in direct ELISAs.
In direct ELISAs and Western blots, no
cross-reactivity with recombinant human UCH-L1 is observed.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Scientific Data Images for Human UCH-L3 Antibody
Detection of Human UCH‑L3 by Western Blot.
Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line and HT-29 human colon adenocarcinoma cell line. PVDF membrane was probed with 3 µg/mL of Mouse Anti-Human UCH-L3 Monoclonal Antibody (Catalog # MAB6008) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for UCH-L3 at approximately 28 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.Applications for Human UCH-L3 Antibody
Application
Recommended Usage
Immunoprecipitation
25 µg/mL
Sample: Cell lysates spiked with Recombinant Human UCH-L3, see our available Western blot detection antibodies
Sample: Cell lysates spiked with Recombinant Human UCH-L3, see our available Western blot detection antibodies
Western Blot
3 µg/mL
Sample: HeLa human cervical epithelial carcinoma cell line and HT‑29 human colon adenocarcinoma cell line
Sample: HeLa human cervical epithelial carcinoma cell line and HT‑29 human colon adenocarcinoma cell line
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: UCH-L3
Long Name
Ubiquitin C-terminal Hydrolase L3
Alternate Names
Ubiquitin Thioesterase L3, UCHL3
Gene Symbol
UCHL3
UniProt
Additional UCH-L3 Products
Product Documents for Human UCH-L3 Antibody
Product Specific Notices for Human UCH-L3 Antibody
For research use only
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