Detects human ULBP‑4/RAET1E in direct ELISAs and Western blots. In Western blots, approximately 20% cross‑reactivity with recombinant human (rh) ULBP-2 is observed and no cross-reactivity with rhULBP‑1 or rhULBP-3 is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG_2B

Applications for Human ULBP-4/RAET1E Alexa Fluor® 750-conjugated Antibody

Application

Recommended Usage

Flow Cytometry

0.25-1 µg/10⁶ cells
Sample: HepG2 human hepatocellular carcinoma cell line

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Formulation

Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: ULBP-4/RAET1E

ULBP-4 (cytomegalovirus glycoprotein UL16 binding protein 4), also called RAET1E (retinoic acid early transcript 1E), Letal (lymphocyte effector cell toxicity activating ligand) and NKG2DL4 (NKG2D ligand 4), is a 40-50 kDa member of the ULBP/RAET1 family of cell surface proteins that function as ligands for NKG2D (1‑6). While most family members are GPI-anchored, only ULBP-4/RAET1E and ULBP-5/RAET1G express a transmembrane form (1, 4, 6, 7). Human ULBP-4 mRNA encodes 263 amino acids (aa) including a 30 aa signal sequence, a 195 aa extracellular domain (ECD), a 23 aa transmembrane domain, and a 15 aa cytoplasmic sequence. A soluble 35 kDa form diverges at aa 208 and is thought to antagonize the transmembrane form (5). Other potential splice variants of 220, 227 and 280 aa are transmembrane proteins (8). Within the ECD, ULBP-4 shares 34-41% aa sequence identity with family members (1, 7). Rodent NKG2D ligands Rae-1 alpha-epsilon are, like human ULBP and MIB proteins, distantly related to MHC class I proteins, but none of the families share significant sequence identity (2, 4). Low expression of ULBP‑4 mRNA is found in normal tissues, with high expression variably reported in the small intestine (3) and skin (4). Expression is stimulated by TNF-alpha and down‑regulated by retinoic acid (3). ULBP-4 is abnormally expressed on most colon cancer and some other tumor cell lines and virus-infected peripheral blood cells (3, 6). ULBP-4 binds and costimulates NKG2D-expressing effector cells including NK cells, NKT cells, gamma delta T cells, and CD8⁺ alpha beta T cells, activating cytolytic activity and/or cytokine production (3, 4, 7). In some gamma delta T cells, direct ULBP-4 binding to both TCR gamma delta and NKG2D has been demonstrated (6). ULBP-4 is also thought to function as a minor histocompatibility antigen in humans (1).

References

Radosavljevic, M. et al. (2002) Genomics 79:114.
Kondo, M. et al. (2010) Immunogenetics 62:441.
Conejo-Garcia, J.R. et al. (2003) Cancer Biol. Ther. 2:446.
Chalupny, N.J. et al. (2003) Biochem. Biophys. Res. Commun. 305:129.
Cao, W. et al. (2007) J. Biol. Chem. 282:18922.
Kong, Y. et al. (2009) Blood 114:310.
Bacon, L. et al. (2004) J. Immunol. 173:1078.
Cao, W. et al. (2008) Int. Immunol. 20:981.

Long Name

UL16 Binding Protein-4

Alternate Names

LETAL, N2DL-4, N2DL4, NKG2DL4, RAET1E, RL-4, ULBP4

Entrez Gene IDs

135250 (Human); 379043 (Mouse); 292461 (Rat)

Gene Symbol

RAET1E

UniProt

Q8TD07 (Human)

Additional ULBP-4/RAET1E Products

Product Documents for Human ULBP-4/RAET1E Alexa Fluor® 750-conjugated Antibody

Product Datasheets

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COA

SDS

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Product Specific Notices for Human ULBP-4/RAET1E Alexa Fluor® 750-conjugated Antibody

This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.

For research use only

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Human ULBP-4/RAET1E Alexa Fluor® 750-conjugated Antibody

R&D Systems, part of Bio-Techne | Catalog # FAB6285S

Key Product Details

Species Reactivity

Validated:

Cited:

Applications