Human Vang-like Protein 1/VANGL1 Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB5476
Key Product Details
Species Reactivity
Validated:
Human
Applications
Immunocytochemistry, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2B Clone # 497707
Product Summary for Human Vang-like Protein 1/VANGL1 Antibody
Immunogen
E. coli-derived recombinant human Vang-like Protein 1/VANGL1
Glu242-Val524
Accession # Q8TAA9
Glu242-Val524
Accession # Q8TAA9
Specificity
Detects human Vang-like Protein 1/VANGL1 in direct ELISAs and Western blots. In direct ELISAs and Western blots, slight cross-reactivity with recombinant human VANGL2 is observed.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2B
Scientific Data Images for Human Vang-like Protein 1/VANGL1 Antibody
Vang-like Protein 1/VANGL1 in HeLa Human Cell Line.
Vang-like Protein 1/VANGL1 was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line using Human Vang-like Protein 1/VANGL1 Monoclonal Antibody (Catalog # MAB5476) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counter-stained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.Applications for Human Vang-like Protein 1/VANGL1 Antibody
Application
Recommended Usage
Immunocytochemistry
8-25 µg/mL
Sample: Immersion fixed HeLa human cervical epithelial carcinoma cell line
Sample: Immersion fixed HeLa human cervical epithelial carcinoma cell line
Western Blot
1 µg/mL
Sample: Recombinant Human Vang-like Protein 1/VANGL1
Sample: Recombinant Human Vang-like Protein 1/VANGL1
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Vang-like Protein 1/VANGL1
Human Van Gogh-like protein 1 (VANGL1; also Vang-like Protein 1, strabismus 2, loop tail protein 2 homolog, LPP2) is a 60 kDa (predicted) member of the Vang family of proteins. VANGL1 is 524 amino acids (aa) in length and contains four transmembrane regions and the C-terminal Ser/Thr-X-Val motif, but lacks a signal sequence. There are two isoforms for VANGL1. In isoform 2, residues 67-68 found in isoform 1 are deleted. Human VANGL1 shares 95% aa sequence identity with mouse VANGL1. The glycoprotein is ubiquitously expressed in normal tissues and is upregulated in many cancer cell lines. VANGL1 is predicted to be a tumor suppressor gene. Mutations in VANGL1 are associated with neural tube defects.
Alternate Names
LPP2, STB2, STBM2, Vang like Protein 1, VANGL1
Gene Symbol
VANGL1
UniProt
Additional Vang-like Protein 1/VANGL1 Products
Product Documents for Human Vang-like Protein 1/VANGL1 Antibody
Product Specific Notices for Human Vang-like Protein 1/VANGL1 Antibody
For research use only
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