Human Vav-1 Antibody
R&D Systems, part of Bio-Techne | Catalog # AF3786
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human
Applications
Validated:
Western Blot
Cited:
Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Sheep IgG
Product Specifications
Immunogen
E. coli-derived recombinant human Vav-1
Trp25-Leu228
Accession # P15498
Trp25-Leu228
Accession # P15498
Specificity
Detects human Vav-1 in Western blots.
Clonality
Polyclonal
Host
Sheep
Isotype
IgG
Scientific Data Images for Human Vav-1 Antibody
Detection of Human Vav-1 by Western Blot.
Western blot shows lysates of Jurkat human acute T cell leukemia cell line and Raji human Burkitt's lymphoma cell line. PVDF membrane was probed with 1 µg/mL of Human Vav-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3786) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Vav-1 at approximately 110 kDa (as indicated). This experiment was conducted using Immunoblot Buffer Group 1.Applications for Human Vav-1 Antibody
Application
Recommended Usage
Western Blot
1 µg/mL
Sample: Jurkat human acute T cell leukemia cell line and Raji human Burkitt's lymphoma cell line
Sample: Jurkat human acute T cell leukemia cell line and Raji human Burkitt's lymphoma cell line
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Vav-1
The Vav-1 proto-oncogene is a guanine nucleotide exchange factor (GEF) for the low molecular weight GTPases of the Rho/Rac family. Vav-1 promotes intracellular signaling by its multiple protein binding motifs. It is a cytosolic protein primarily expressed in hematopoietic cells and plays an essential role in the proliferation and activation of T and B cells.
Alternate Names
vav-T, Vav1
Gene Symbol
VAV1
UniProt
Additional Vav-1 Products
Product Documents for Human Vav-1 Antibody
Product Specific Notices for Human Vav-1 Antibody
For research use only
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